中华消化杂志
中華消化雜誌
중화소화잡지
Chinese Journal of Digestion
2010年
3期
184-188
,共5页
金海林%施瑞华%朱宏%张伟锋%曾楷峰%郝波
金海林%施瑞華%硃宏%張偉鋒%曾楷峰%郝波
금해림%시서화%주굉%장위봉%증해봉%학파
食管肿瘤%缺氧诱导因子-1%EphA2%RNA干扰%转染
食管腫瘤%缺氧誘導因子-1%EphA2%RNA榦擾%轉染
식관종류%결양유도인자-1%EphA2%RNA간우%전염
Esophageal neoplasms%Hypoxia-inducible factor-1α%EphA2%RNA interference%Transfection
目的 检测缺氧诱导因子-1α (HIF-1α) 蛋白与EphA2蛋白在食管鳞状细胞癌中的表达定位并探讨二者表达的相关性.方法 采用细胞免疫荧光法检测HIF-1α与EphA2在食管鳞状细胞癌细胞株Eca109中的表达与定位;采用免疫组化法检测HIF-1α与EphA2在41例食管鳞状细胞癌和25例正常食管组织中的表达;常氧和缺氧条件下,Western 印迹法检测食管鳞状细胞癌细胞株Eca109和TE13经RNA干扰(RNAi)技术特异性沉默HIF-1α后EphA2表达的变化.结果 ①细胞免疫荧光结果显示HIF-1α和EphA2均在Eca109细胞胞质表达.②免疫组化结果显示HIF-1α在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为70.73%(29/41)和0%(0/25),两者比较差异有统计学意义(P<0.05).EphA2在食管鳞状细胞癌和正常食管组织中的阳性表达率分别为78.04%(32/41)和28%(7/25),两者比较差异有统计学意义(P<0.05).相关性检验提示HIF-1α和EphA2在食管鳞状细胞癌中表达呈正相关性(r=0.5654,χ~2=13.11,P<0.05).③ Western印迹结果显示在食管鳞状细胞癌细胞株Eca109和TE13中,EphA2表达随HIF-1α的沉默而受抑制.结论 HIF-1α与EphA2在食管鳞状细胞癌组织中呈高表达,且二者表达呈正相关;EphA2表达受HIF-1α的调控,可能为HIF-1α的靶基因.
目的 檢測缺氧誘導因子-1α (HIF-1α) 蛋白與EphA2蛋白在食管鱗狀細胞癌中的錶達定位併探討二者錶達的相關性.方法 採用細胞免疫熒光法檢測HIF-1α與EphA2在食管鱗狀細胞癌細胞株Eca109中的錶達與定位;採用免疫組化法檢測HIF-1α與EphA2在41例食管鱗狀細胞癌和25例正常食管組織中的錶達;常氧和缺氧條件下,Western 印跡法檢測食管鱗狀細胞癌細胞株Eca109和TE13經RNA榦擾(RNAi)技術特異性沉默HIF-1α後EphA2錶達的變化.結果 ①細胞免疫熒光結果顯示HIF-1α和EphA2均在Eca109細胞胞質錶達.②免疫組化結果顯示HIF-1α在食管鱗狀細胞癌和正常食管組織中的暘性錶達率分彆為70.73%(29/41)和0%(0/25),兩者比較差異有統計學意義(P<0.05).EphA2在食管鱗狀細胞癌和正常食管組織中的暘性錶達率分彆為78.04%(32/41)和28%(7/25),兩者比較差異有統計學意義(P<0.05).相關性檢驗提示HIF-1α和EphA2在食管鱗狀細胞癌中錶達呈正相關性(r=0.5654,χ~2=13.11,P<0.05).③ Western印跡結果顯示在食管鱗狀細胞癌細胞株Eca109和TE13中,EphA2錶達隨HIF-1α的沉默而受抑製.結論 HIF-1α與EphA2在食管鱗狀細胞癌組織中呈高錶達,且二者錶達呈正相關;EphA2錶達受HIF-1α的調控,可能為HIF-1α的靶基因.
목적 검측결양유도인자-1α (HIF-1α) 단백여EphA2단백재식관린상세포암중적표체정위병탐토이자표체적상관성.방법 채용세포면역형광법검측HIF-1α여EphA2재식관린상세포암세포주Eca109중적표체여정위;채용면역조화법검측HIF-1α여EphA2재41례식관린상세포암화25례정상식관조직중적표체;상양화결양조건하,Western 인적법검측식관린상세포암세포주Eca109화TE13경RNA간우(RNAi)기술특이성침묵HIF-1α후EphA2표체적변화.결과 ①세포면역형광결과현시HIF-1α화EphA2균재Eca109세포포질표체.②면역조화결과현시HIF-1α재식관린상세포암화정상식관조직중적양성표체솔분별위70.73%(29/41)화0%(0/25),량자비교차이유통계학의의(P<0.05).EphA2재식관린상세포암화정상식관조직중적양성표체솔분별위78.04%(32/41)화28%(7/25),량자비교차이유통계학의의(P<0.05).상관성검험제시HIF-1α화EphA2재식관린상세포암중표체정정상관성(r=0.5654,χ~2=13.11,P<0.05).③ Western인적결과현시재식관린상세포암세포주Eca109화TE13중,EphA2표체수HIF-1α적침묵이수억제.결론 HIF-1α여EphA2재식관린상세포암조직중정고표체,차이자표체정정상관;EphA2표체수HIF-1α적조공,가능위HIF-1α적파기인.
Objective To investigate the expressions and the correlation of hypoxia-inducible factor(HIF)-1α and EphA2 in esophageal squamous cell carcinoma.Methods The subcellular localizations of HIF-1α and EphA2 in Eca109 cell line were characterized by immunofluorescence staining.The expressions of HIF-1α and EphA2 in human esophageal squamous cell carcinoma tissues were detected by immunohistochemistry.Western blot was used to detect the expression of EphA2 in Eca109 cell line with knock-down HIF-1α gene and in TEl3 cell line under normoxia and hypoxia in
vitro.Results ① HIF-1α and EphA2 could be detected in cytoplasm in Eca109.② The positive rates of HIF-1α and EphA2 were significantly higher in esophageal squamous cell carcinoma tissues than those in normal esophagus tissues (70.73% vs 0%,P<0.05;78.04% vs 28%,P<0.05).The correlation analysis indicated that the expression of EphA2 had a positive correlation with that of HIF-1α in esophageal squamous eell carcinoma.③ Western blot showed that the expression of EphA2 in
Eca109 and TE13 was inhibited with silencing HIF-1α.Conclusions HIF-1α and EphA2 are overexpressed in esophageal squamous cell carcinoma tissues.The expression of EphA2 protein positively correlates with expression of HIF-1α protein.EphA2 may be a potential target of HIF-1α.
