中华肿瘤杂志
中華腫瘤雜誌
중화종류잡지
CHINESE JOURNAL OF ONCOLOGY
2009年
7期
490-494
,共5页
黄川%樊晓晖%姜艳华%宋德志%高灵茜%黄企光%赖振屏
黃川%樊曉暉%薑豔華%宋德誌%高靈茜%黃企光%賴振屏
황천%번효휘%강염화%송덕지%고령천%황기광%뢰진병
新城疫病毒%溶瘤病毒%结肠肿瘤%小鼠,裸
新城疫病毒%溶瘤病毒%結腸腫瘤%小鼠,裸
신성역병독%용류병독%결장종류%소서,라
Newcastle disease virus%Oncolytic viruses%Colonic neoplasms%Mouse,nude
目的 观察新城疫病毒DK/HK/817/1980(NDV D817)对裸小鼠人结肠癌移植瘤的抑制作用.方法 采用LoVo细胞株建立裸小鼠人结肠癌移植瘤模型,分别给予裸小鼠尾静脉注射PBS、氟尿嘧啶(5-Fu)以及NDV D817高、中、低3个剂量,观察不同剂量NDV D817对移植瘤的抑制作用,病理学观察NDV D817对移植瘤和肝细胞的损伤程度,流式细胞术检测肿瘤细胞的凋亡和坏死情况,酶联免疫吸附试验(ELISA)试剂盒检测荷瘤小鼠体内肿瘤坏死因子-α(TNF-α)的含量,血凝实验检测荷瘤小鼠体内活病毒量.结果 中剂量NDV D817可明显抑制移植瘤的生长,肿瘤抑制率达48.1%.NDV D817对荷瘤小鼠体重和肝脏的损伤较小,且具有诱导肿瘤细胞凋亡和诱导机体产生TNF-α的能力.荷瘤小鼠处死后只在瘤内检测到活病毒,而在血清和其他脏器中并没有检出活病毒.结论 在对裸小鼠人结肠癌移植瘤的抑制过程中,NDV D817有明显的抑瘤效果,适当剂量的NDVD817更安全有效.
目的 觀察新城疫病毒DK/HK/817/1980(NDV D817)對裸小鼠人結腸癌移植瘤的抑製作用.方法 採用LoVo細胞株建立裸小鼠人結腸癌移植瘤模型,分彆給予裸小鼠尾靜脈註射PBS、氟尿嘧啶(5-Fu)以及NDV D817高、中、低3箇劑量,觀察不同劑量NDV D817對移植瘤的抑製作用,病理學觀察NDV D817對移植瘤和肝細胞的損傷程度,流式細胞術檢測腫瘤細胞的凋亡和壞死情況,酶聯免疫吸附試驗(ELISA)試劑盒檢測荷瘤小鼠體內腫瘤壞死因子-α(TNF-α)的含量,血凝實驗檢測荷瘤小鼠體內活病毒量.結果 中劑量NDV D817可明顯抑製移植瘤的生長,腫瘤抑製率達48.1%.NDV D817對荷瘤小鼠體重和肝髒的損傷較小,且具有誘導腫瘤細胞凋亡和誘導機體產生TNF-α的能力.荷瘤小鼠處死後隻在瘤內檢測到活病毒,而在血清和其他髒器中併沒有檢齣活病毒.結論 在對裸小鼠人結腸癌移植瘤的抑製過程中,NDV D817有明顯的抑瘤效果,適噹劑量的NDVD817更安全有效.
목적 관찰신성역병독DK/HK/817/1980(NDV D817)대라소서인결장암이식류적억제작용.방법 채용LoVo세포주건립라소서인결장암이식류모형,분별급여라소서미정맥주사PBS、불뇨밀정(5-Fu)이급NDV D817고、중、저3개제량,관찰불동제량NDV D817대이식류적억제작용,병이학관찰NDV D817대이식류화간세포적손상정도,류식세포술검측종류세포적조망화배사정황,매련면역흡부시험(ELISA)시제합검측하류소서체내종류배사인자-α(TNF-α)적함량,혈응실험검측하류소서체내활병독량.결과 중제량NDV D817가명현억제이식류적생장,종류억제솔체48.1%.NDV D817대하류소서체중화간장적손상교소,차구유유도종류세포조망화유도궤체산생TNF-α적능력.하류소서처사후지재류내검측도활병독,이재혈청화기타장기중병몰유검출활병독.결론 재대라소서인결장암이식류적억제과정중,NDV D817유명현적억류효과,괄당제량적NDVD817경안전유효.
Objective To study the anti-tumor effects of Newcastle disease virus (NDV) strain D817 on human colon carcinoma model in nude mice. Methods The nude mouse model of human colon carcinoma was established by subcutaneous inoculation of human colon cancer LOVO cells. The tumor-bearing mice were given PBS, 5-Fu, high-dose NDV D817, moderate-dese NDV D817 or low-dose NDV D817 via caudal vein injection. The tumor size and weight of mice were measured. The liver damages were examined by histopathology. Apoptosis and necrosis of tumor ceils were detected by flow cytometry. The endotumoral content of TNF-α was detected using a mouse TNF-a ELISA kit. The live vires was detected by bemagglutination (HA) test. Results The moderate-dose NDV D817 inhibited the tumor growth more apparently than 5-Fu. The tumor growth inhibition rate reached to 48.1%. The liver damage and the weight change caused by NDV were less severe. NDV D817 made an increased apoptosis index and induced production of TNF-α. Live virus was not detected in important organs except in the tumor of nude mice by HA test. Conclusion In the anti-tumor process in nude mice bearing xenografts of human colon carcinoma, a suitable dose of NDV D817 is more safe and effective.
