四川大学学报(医学版)
四川大學學報(醫學版)
사천대학학보(의학판)
JOURNAL OF WEST CHINA UNIVERSITY OF MEDICAL SCIENCES
2010年
1期
137-139,152
,共4页
应激%抑郁%大鼠%强迫游泳
應激%抑鬱%大鼠%彊迫遊泳
응격%억욱%대서%강박유영
Stress%Depression%Rat%Forced swimming
目的 比较两种慢性强迫游泳应激抑郁模型的行为学特点,探讨慢性强迫游泳应激抑郁模型的建立方法,方法 18只SD雄性大鼠随机分为对照组和应激组(S1组和S2组).应激组连续给予14 d、水温分别为(23±0.5)℃和(10±0.5)℃、每天5 min强迫游泳,观察3组大鼠应激7 d和14 d的体质量、摄食、旷场实验和液体消耗实验的变化.结果 S2组在应激7 d后体质量增长率、24 h摄食率低于对照组(P＜0.05);应激14 d后体质量增长率[(12.26±4.04)%]、24 h摄食率((9.49±0.96)%]、摄糖量[(28.63±3.51)g]、糖水偏好[(76.25±2.51)%]、水平得分(12.17±9.00)、垂直得分(3.17±3.60)均低于对照组(P＜0.0S).S1组在应激7 d后出现体质量增长率、24 h摄食率明显下降,在应激14 d后除体质量增长率下降外,24 h摄食率、垂直得分和糖水偏好均高于对照组(P＜0.05).结论 较低水温的慢性强迫游泳应激14 d能诱发大鼠的抑郁行为,可能更适合于建立应激抑郁模型.
目的 比較兩種慢性彊迫遊泳應激抑鬱模型的行為學特點,探討慢性彊迫遊泳應激抑鬱模型的建立方法,方法 18隻SD雄性大鼠隨機分為對照組和應激組(S1組和S2組).應激組連續給予14 d、水溫分彆為(23±0.5)℃和(10±0.5)℃、每天5 min彊迫遊泳,觀察3組大鼠應激7 d和14 d的體質量、攝食、曠場實驗和液體消耗實驗的變化.結果 S2組在應激7 d後體質量增長率、24 h攝食率低于對照組(P＜0.05);應激14 d後體質量增長率[(12.26±4.04)%]、24 h攝食率((9.49±0.96)%]、攝糖量[(28.63±3.51)g]、糖水偏好[(76.25±2.51)%]、水平得分(12.17±9.00)、垂直得分(3.17±3.60)均低于對照組(P＜0.0S).S1組在應激7 d後齣現體質量增長率、24 h攝食率明顯下降,在應激14 d後除體質量增長率下降外,24 h攝食率、垂直得分和糖水偏好均高于對照組(P＜0.05).結論 較低水溫的慢性彊迫遊泳應激14 d能誘髮大鼠的抑鬱行為,可能更適閤于建立應激抑鬱模型.
목적 비교량충만성강박유영응격억욱모형적행위학특점,탐토만성강박유영응격억욱모형적건립방법,방법 18지SD웅성대서수궤분위대조조화응격조(S1조화S2조).응격조련속급여14 d、수온분별위(23±0.5)℃화(10±0.5)℃、매천5 min강박유영,관찰3조대서응격7 d화14 d적체질량、섭식、광장실험화액체소모실험적변화.결과 S2조재응격7 d후체질량증장솔、24 h섭식솔저우대조조(P＜0.05);응격14 d후체질량증장솔[(12.26±4.04)%]、24 h섭식솔((9.49±0.96)%]、섭당량[(28.63±3.51)g]、당수편호[(76.25±2.51)%]、수평득분(12.17±9.00)、수직득분(3.17±3.60)균저우대조조(P＜0.0S).S1조재응격7 d후출현체질량증장솔、24 h섭식솔명현하강,재응격14 d후제체질량증장솔하강외,24 h섭식솔、수직득분화당수편호균고우대조조(P＜0.05).결론 교저수온적만성강박유영응격14 d능유발대서적억욱행위,가능경괄합우건립응격억욱모형.
Objective To compare the behaviors of rats with depressions induced by chronic forced swimming stress under two different conditions.Methods Eighteen male rats were randomly divided into 3 groups,with 6 rats in each group.The rats in the control group(C group)were not forced into swimming,while the rats in the stress groups(S1 and S2)were forced to swim for 14 consecutive days.The rats in S1 group and S2 group swam for five minutes every morning,in water with(23±1)℃,and(10±0.5)℃ in temperature,respectively.The weight gain,food intake,open-field test and saccharin solution test were observed on the seventh day and fourteenth day.Results On the seventh day following chronic swim stress,the rats in the S2 group had significant lower ratio in weight gain and food intake than the controls(P＜0.05).On the fourteenth day,the rats in the S2 group had significant lower ratio in weight gain(12.26±4.04)%,food intake(9.49±0.96)%,sucrose intake(28.63±3.51)g,and preference for saccharin solution(76.25±2.51)%,and less number of crossing(12.17±9.00)and times of rearing(3.17±3.60)than the controls(P＜0.05).The rats in the S1 group had significant lower ratio in weight gain and food intake than the controls on the seventh day following forced swimming.On the fourteenth day,the rats in the S1 group still had lower ratio in weight gain,but had higher ratio in food intake and preference for saccharin solution,and greater number of crossing than the controls.Conclusion Chronic forced swimming at a lower temperature could induce depression better than at a higher temperature.
