CAJ | 학술논문

目的:研究香椿叶提取物抗氧化和抑制细胞增殖的活性.方法:借助溶剂浸提和聚酰胺树脂获得香椿叶的总酚提取物进而考察其含量.采用TOSC实验衡量香椿叶提取物的总抗氧化活性,同时利用体外实验观察其对人类不同类型癌细胞生长的抑制作用.结果:香椿叶提取物的总酚含量为(427.53±4.31) mg/g,每克样品的抗氧化活性达到807.64μmoL维生素C当量.提取物可以显著抑制人肠癌细胞Caco-2、肝癌细胞HepG2和乳腺癌细胞MCF-7的生长,其EC50分别为(4.00±0.39),(153.16±13.49),(193.46±14.68) μg/mL.香椿叶提取物的生物活性指数(BI)约283,肠癌细胞Caco-2对提取物的敏感性超过了乳腺癌细胞MCF-7和肝癌细胞HepG2.结论:香椿叶提取物具有一定抗癌功效,对慢性疾病的预防具有应用价值.
목적:연구향춘협제취물항양화화억제세포증식적활성.방법:차조용제침제화취선알수지획득향춘협적총분제취물진이고찰기함량.채용TOSC실험형량향춘협제취물적총항양화활성,동시이용체외실험관찰기대인류불동류형암세포생장적억제작용.결과:향춘협제취물적총분함량위(427.53±4.31) mg/g,매극양품적항양화활성체도807.64μmoL유생소C당량.제취물가이현저억제인장암세포Caco-2、간암세포HepG2화유선암세포MCF-7적생장,기EC50분별위(4.00±0.39),(153.16±13.49),(193.46±14.68) μg/mL.향춘협제취물적생물활성지수(BI)약283,장암세포Caco-2대제취물적민감성초과료유선암세포MCF-7화간암세포HepG2.결론:향춘협제취물구유일정항암공효,대만성질병적예방구유응용개치.
Objective:To determine the antioxidization and antiproliferation of extract from leaves of Toona sinensis (LTS).Methods:The total phenolic extract of LTS was obtained by solvent and polyamide resin to determine the content.The antioxidization of the LTS extract was measured by TOSC assay.Antiproliferation was studied in vitro with different human cancer cells.Results:The total phenolic content in the LTS was (427.53±4.31) mg/g and antioxidization was 807.64 μmoL vitamin C equivalents/g in the sample.The extract significantly inhibited the colon cancer cell Caco-2,human liver cancer cell HepG2 and breast cancer cell MCF-7 proliferation with EC50 (4.00±0.39),(153.16±13.49) and (193.46±14.68) μg/mL,respectively.The bioactivity index (BI) of the LTS extract was nearly 283.Caco-2 was more sensitive than MCF-7 and HepG2.Conclusion:Extract from LTS has anticancer properties useful for preventing chronic diseases.