CAJ | 학술논문

目的研究肝肠粘连蛋白(liver intestine cadherin,LI-cadherin)在荷瘤裸鼠体内对肝癌细胞Hep3B侵袭转移能力的作用.方法体外培养肝癌细胞株Hep3B,并进行针对LI-cadherin的SiRNA转染.将Hep3B细胞及转染后的肿瘤细胞接种入裸鼠脾脏,建立裸鼠荷瘤模型.观察成瘤及转移效果,免疫组化检测转移灶组织结构,Western blot方法检测转染前后荷瘤裸鼠体内肿瘤转移灶中LI-cadherin的表达.结果 (1)SiRNA质粒转染入Hep3B肝癌细胞,转染率达到80％.(2)建立裸鼠荷人肝癌模型,30只裸鼠移植手术后均成活,荷瘤率达到60％以上.SiRNA转染组荷瘤率为80％,转移灶个数为26个,明显高于空白对照组和空质粒转染组.(3)裸鼠体内转移灶中Ll-cadherin 的含量SiRNA转染组明显低于空白对照组和空质粒转染组.结论 LI-cadherin对肝癌细胞的黏附能力有重要作用,对LI-cadherin进行SiRNA干扰可增强肝细胞癌在裸鼠体内的侵袭转移能力.
목적 연구간장점련단백(liver intestine cadherin,LI-cadherin)재하류라서체내대간암세포Hep3B침습전이능력적작용.방법 체외배양간암세포주Hep3B,병진행침대LI-cadherin적SiRNA전염.장Hep3B세포급전염후적종류세포접충입라서비장,건립라서하류모형.관찰성류급전이효과,면역조화검측전이조조직결구,Western blot방법검측전염전후하류라서체내종류전이조중LI-cadherin적표체.결과 (1)SiRNA질립전염입Hep3B간암세포,전염솔체도80％.(2)건립라서하인간암모형,30지라서이식수술후균성활,하류솔체도60％이상.SiRNA전염조하류솔위80％,전이조개수위26개,명현고우공백대조조화공질립전염조.(3)라서체내전이조중Ll-cadherin 적함량SiRNA전염조명현저우공백대조조화공질립전염조.결론 LI-cadherin대간암세포적점부능력유중요작용,대LI-cadherin진행SiRNA간우가증강간세포암재라서체내적침습전이능력.
Objective To investigate the effect of LI-cadherin- SiRNA protein on the growth and metastatic potentials of transplanted human hepatocellular carcinoma cell lines (Hep3B) in nude mice.Methods We transfected LI-cadherin- SiRNA to Hep3B cells,Hep3B cell suspension (transfected or control ) was injected subcapsullaryly into the spleen of nude mice,hepatic metastasis was observed by naked eye and immunohistochemistry.In addition,Western-blot was used to detect the level of LI-cadherin in different metastatic site.Results (1) Hep3B was green after successful transfect interference vector under fluorescence inverted microscope,in this study,the transfect rate was 80％.(2) Hep3B liver metastasis model in nude mice was established.The metastasis rates in the empty plasmid carrying group,the control group and the SiRNA transfect group were 50％,60％ and 80％,respectively.The number of metastasis caner nodules in the SiRNA transfect group was 26,significantly higher than other two groups.(3) The level of protein expression for LI-cadherin in the SiRNA transfect group is significantly lower than the control group and the empty plasmid carrying group.Conclusions LI-cadherin is crucial and important for the adhension capability of HCC in its migration.SiRNA transfected LI-cadherin increases the metastasis in a nude mouse model inoculated with human hepatocellular carcinoma cell lines.