CAJ | 학술논문

目的初步探讨半乳糖基白蛋白-超顺磁性氧化铁(Gal-BSA-SPIO)在肝脏小肿瘤检出中的应用价值.方法用半乳糖基白蛋白包被小粒径超顺磁性氧化铁(SPIO)制备Gal-BSA-SPIO,测定其粒径大小.建立兔VX2肝癌模型(肿瘤直径＜1.5 cm),用数字表法随机分为Gal-BSA-SPIO和SPIO组,每组10只,每组再分为5和10 μmol Fe/kg组,每组5只.所有实验动物均分别采用SE T2WI、快速自旋回波(FSE)T2WI和梯度回波(GRE)T2WI序列行MR平扫,并按实验分组行增强扫描,分别测量增强前后兔肝脏信噪比(SNR)和肿瘤与肝脏对比噪声比(CNR),并采用方差分析进行比较.扫描完毕后对肝脏及肿瘤组织进行病理检查.结果 Gal-BSA-SPIO平均粒径为34.4 nm,核心粒径为14.8 nm.5 μmol Fe/kg SPIO、5 μmol Fe/kg Gal-BSA-SPIO、10 μmol Fe/kg SPIO和10 μmol Fe/kg Gal-BSA-SPIO 4组增强前后肝脏SNR差值SE序列分别为(1.92±0.42)、(3.75±0.51)、(3.25±0.72)、(4.73±0.34),FSE序列分别为(1.56±0.18)、(3.56±0.64)、(3.33±0.16)、(4.83±0.16),GRE序列分别为(5.55±0.91)、(9.58±0.66)、(9.20±0.29)、(10.63±0.32),不同对比剂及不同序列组间比较差异均有统计学意义(P值均＜0.01).上述4组增强前后肿瘤与肝脏CNR差值SE序列分别为(2.22±0.68)、(5.27±0.22)、(4.25±0.51)、(6.97±0.63),FSE序列分别为(1.73±0.41)、(4.46±0.54)、(3.73±0.49)、(5.39±0.39),GRE序列分别为(5.98±0.71)、(11.79±0.57)、(9.57±0.54)、(14.64±1.35),不同对比剂及不同序列组间比较差异均有统计学意义(P值均＜0.01).Gal-BSA-SPIO主要作用于肝实质细胞,而SPIO主要分布于Kupffer细胞内.结论 Gal-BSA-SPIO具有良好的负向肝脏强化效应,对肝内小肿瘤的检出具有良好的潜在应用价值.
목적 초보탐토반유당기백단백-초순자성양화철(Gal-BSA-SPIO)재간장소종류검출중적응용개치.방법 용반유당기백단백포피소립경초순자성양화철(SPIO)제비Gal-BSA-SPIO,측정기립경대소.건립토VX2간암모형(종류직경＜1.5 cm),용수자표법수궤분위Gal-BSA-SPIO화SPIO조,매조10지,매조재분위5화10 μmol Fe/kg조,매조5지.소유실험동물균분별채용SE T2WI、쾌속자선회파(FSE)T2WI화제도회파(GRE)T2WI서렬행MR평소,병안실험분조행증강소묘,분별측량증강전후토간장신조비(SNR)화종류여간장대비조성비(CNR),병채용방차분석진행비교.소묘완필후대간장급종류조직진행병리검사.결과 Gal-BSA-SPIO평균립경위34.4 nm,핵심립경위14.8 nm.5 μmol Fe/kg SPIO、5 μmol Fe/kg Gal-BSA-SPIO、10 μmol Fe/kg SPIO화10 μmol Fe/kg Gal-BSA-SPIO 4조증강전후간장SNR차치SE서렬분별위(1.92±0.42)、(3.75±0.51)、(3.25±0.72)、(4.73±0.34),FSE서렬분별위(1.56±0.18)、(3.56±0.64)、(3.33±0.16)、(4.83±0.16),GRE서렬분별위(5.55±0.91)、(9.58±0.66)、(9.20±0.29)、(10.63±0.32),불동대비제급불동서렬조간비교차이균유통계학의의(P치균＜0.01).상술4조증강전후종류여간장CNR차치SE서렬분별위(2.22±0.68)、(5.27±0.22)、(4.25±0.51)、(6.97±0.63),FSE서렬분별위(1.73±0.41)、(4.46±0.54)、(3.73±0.49)、(5.39±0.39),GRE서렬분별위(5.98±0.71)、(11.79±0.57)、(9.57±0.54)、(14.64±1.35),불동대비제급불동서렬조간비교차이균유통계학의의(P치균＜0.01).Gal-BSA-SPIO주요작용우간실질세포,이SPIO주요분포우Kupffer세포내.결론 Gal-BSA-SPIO구유량호적부향간장강화효응,대간내소종류적검출구유량호적잠재응용개치.
Objective To discuss the value of detection of small liver tumor with Galactose-Bovine-serum-albumin containing superparamagetic(Gal-BSA-SPIO) in rabbits primarily.Methods Gal-BSA-SPIO was obtained by stabilizing small superparamagnetic iron with galactose-bovine-serum-albumin,and its size was measured.Models of liver tumor(diameter＜1.5 cm) in rabbits were founded.The animals were randomly divided into groups of SPIO (n=10) and group of Gal-BSA-SPIO (n=10).Each group was further subdivided as 5 μmol Fe/kg group (n=5) and 10 μmol Fe/kg group (n=5),respectively.All animals were performed magnetic resonance imaging before and after administration of agents according to groups.SE T2WI,FSE T2WI and GRE T2WI sequences were used.Then signal of noise(SNR) of liver and contrast-noise ratio(CNR) of tumor-liver were measured and compared statistically.The histopathology study was performed for liver and tumor specimens after MR imaging.Results The mean value for the volume size was 34.4 nm,and the core particle size was 14.8 nm.The differences of liver SNR between before and after enhancement for 5 μmol Fe/kg SPIO,5 μmol Fe/kg Gal-BSA-SPIO,10 μmol Fe/kg SPIO and 10 μmol Fe/kg Gal-BSA-SPIO group were (1.92±0.42),(3.75±0.51),(3.25±0.72) and (4.73±0.34) on SE sequence,respectively;(1.56±0.18),(3.56±0.64),(3.33±0.16) and (4.83±0.16) on FSE sequence,respectively;(5.55±0.91),(9.58±0.66),(9.20±0.29) and (10.63±0.32) on GRE sequence,respectively.There were significant differences between all groups for difierent doses and agents and within all different sequences (P＜0.01).The differences of tumor-liver CNR between before and after enhancement of these 4 groups were(2.22±0.68),(5.27±0.22),(4.25±0.51) and (6.97±0.63) on SE sequence,respectively;(1.73±0.41),(4.46±0.54),(3.73±0.49)and (5.39±0.39) on FSE sequence,respectively;(5.98±0.71),(11.79±0.57),(9.57±0.54)and(14.64±1.35)on GRE sequence,respectively.The differences between all groups of different doses and agents and within all sequences showed significant (P＜0.01). Histopathological study revealed that Gal-BSA-SPIO was mainly distributed in hepatocytes while SPIO mainly in Kupffer cells.Conclusion Gal-BSA-SPIO is a agent with good negative enhancement effect in liver.It may be helpful for detecting small tumor of liver.