中华麻醉学杂志
中華痳醉學雜誌
중화마취학잡지
CHINESE JOURNAL OF ANESTHESIOLOGY
2011年
6期
699-701
,共3页
吴焕兵%赵邦娥%张登文%夏辉%田学愎%田玉科%王学仁
吳煥兵%趙邦娥%張登文%夏輝%田學愎%田玉科%王學仁
오환병%조방아%장등문%하휘%전학퍅%전옥과%왕학인
疼痛,手术后%KATP通道%脊髓
疼痛,手術後%KATP通道%脊髓
동통,수술후%KATP통도%척수
Pain,postoperative%KATP channels%Spinal cord
目的 评价开胸术后慢性痛大鼠脊髓背角神经元ATP敏感型钾通道(KATP通道)表达的变化.方法 雄性SD大鼠20只,体重250~350 g,采用随机数字表法,将其随机分为假手术组和开胸术后慢性痛组,每组10只.于术前1 d、术后1、5、10、15、20、25、30 d时分别测定机械痛阈,并行冷丙酮实验,记录1 min内大鼠后爪抓挠皮区次数;于术后30d时处死大鼠,取颈至上胸段脊髓,免疫组化法检测KATP通道的表达水平.结果 与假手术组比较,开胸术后慢性痛组大鼠术后5~如d时机械痛阈降低,术后20~30d时后爪抓挠皮区次数增加,术后30 d时胸段脊髓背角神经元KATP通道表达下调(P<0.05).结论 开胸术后慢性痛大鼠脊髓背角神经元KATP通道表达下调,该变化可能参与了开胸术后慢性痛的病理生理机制.
目的 評價開胸術後慢性痛大鼠脊髓揹角神經元ATP敏感型鉀通道(KATP通道)錶達的變化.方法 雄性SD大鼠20隻,體重250~350 g,採用隨機數字錶法,將其隨機分為假手術組和開胸術後慢性痛組,每組10隻.于術前1 d、術後1、5、10、15、20、25、30 d時分彆測定機械痛閾,併行冷丙酮實驗,記錄1 min內大鼠後爪抓撓皮區次數;于術後30d時處死大鼠,取頸至上胸段脊髓,免疫組化法檢測KATP通道的錶達水平.結果 與假手術組比較,開胸術後慢性痛組大鼠術後5~如d時機械痛閾降低,術後20~30d時後爪抓撓皮區次數增加,術後30 d時胸段脊髓揹角神經元KATP通道錶達下調(P<0.05).結論 開胸術後慢性痛大鼠脊髓揹角神經元KATP通道錶達下調,該變化可能參與瞭開胸術後慢性痛的病理生理機製.
목적 평개개흉술후만성통대서척수배각신경원ATP민감형갑통도(KATP통도)표체적변화.방법 웅성SD대서20지,체중250~350 g,채용수궤수자표법,장기수궤분위가수술조화개흉술후만성통조,매조10지.우술전1 d、술후1、5、10、15、20、25、30 d시분별측정궤계통역,병행랭병동실험,기록1 min내대서후조조뇨피구차수;우술후30d시처사대서,취경지상흉단척수,면역조화법검측KATP통도적표체수평.결과 여가수술조비교,개흉술후만성통조대서술후5~여d시궤계통역강저,술후20~30d시후조조뇨피구차수증가,술후30 d시흉단척수배각신경원KATP통도표체하조(P<0.05).결론 개흉술후만성통대서척수배각신경원KATP통도표체하조,해변화가능삼여료개흉술후만성통적병리생리궤제.
Objective To evaluate the change in the expression of ATP-sensitive potassium (KATP) channels in spinal dorsal horn neurons in rats with chronic post-thoracotomy pain (CPTP). Methods Twenty male SD rats, weighing 250-350 g, were randomly divided into 2 groups ( n = 10 each): sham operation group and CPTP group. The animals were anesthetized with 10% chloral hydrate 300 mg/kg, tracheostomized and mechanically ventilated. At 1 d before operation, and at 1, 5, 10, 15, 20, 25 and 30 d after operation, paw withdrawal threshold to yon Frey filament stimulation was measured and the acetone test was performed. The responses of the hindpaw to the acetone test within 1 min were recorded. The rats were sacrificed at 30 d after operation, and the cervical and upper thoracic segments of the spinal cord were removed to detect the expression of KATP channels in spinal dorsal horn neurons by immuno-histochemistry. Results Compared with sham operation group, mechanical pain threshold was significantly decreased at 5-30 d after operation, the response frequency was significantly increased at 20-30 d after operstion, and the expression of KATP channels in spinal dorsal horn neurons in the thoracic segments was down-regulsted at 30 d after operation in CPTP group ( P < 0.05). Conclusion The expression of KATP channels in spinal dorsal horn neurons is down-regulated in rots with CPTP,which may be involved in the pathophsiology mechanism of CPTP.