CAJ | 학술논문

目的探讨早产儿视网膜病变(ROP)易感性与血管内皮生长因子(VEGF)-A+ 405和VEGF-A936基因多态性的相关性。方法 99例ROP患儿（ROP组）和80例非ROP早产儿（对照组）纳入本研究。两组受检者的出生年龄、出生体重及给氧时间之间差异均无统计学意义(P＞0.05)。取得所有受检者监护人的知情同意后，抽取外周静脉血2 ml。采用焦磷酸测序法检测VEGF-A+ 405和VEGF-A936基因多态性表型、等位基因和基因频率。分析ROP与VEGF-A+ 405和VEGF-A936基因多态性的相关性。结果 VEGF-A+ 405存在CC、GG、CG 3种基因型，VEGF-A936存在CC、CT 2种基因型。ROP组VEGF-A+405位点C、G等位基因分别为92、106，基因频率分别为46.5％，53.5％；对照组VEGF-A+405位点C、G等位基因分别为90、70，基因频率分别为56.2％，43.8％；两组比较，差异无统计学意义(X2=3.396，P=0.066)。相关性分析发现，VEGF-A+ 405基因多态性与ROP发生无相关性(OR=0.675，OR95％ CI=0.444，1.026)。ROP组VEGF-A936位点T、C等位基因分别为32、166，基因频率分别为16.2％，83.8％；对照组VEGF-A936位点T、C等位基因分别为16、144，基因频率分别为1o.o％，90.0％；两组比较，差异无统计学意义(X2 =2.894，P=0.089)。相关性分析发现，VEGF-A936基因多态性与ROP发生无相关性(OR=0.768，OR95％ CI=0.711，0.829)。结论 VEGF-A+ 405和VEGF-A936基因多态性与ROP易感性无关。
목적 탐토조산인시망막병변(ROP)역감성여혈관내피생장인자(VEGF)-A+ 405화VEGF-A936기인다태성적상관성。방법 99례ROP환인（ROP조）화80례비ROP조산인（대조조）납입본연구。량조수검자적출생년령、출생체중급급양시간지간차이균무통계학의의(P＞0.05)。취득소유수검자감호인적지정동의후，추취외주정맥혈2 ml。채용초린산측서법검측VEGF-A+ 405화VEGF-A936기인다태성표형、등위기인화기인빈솔。분석ROP여VEGF-A+ 405화VEGF-A936기인다태성적상관성。결과 VEGF-A+ 405존재CC、GG、CG 3충기인형，VEGF-A936존재CC、CT 2충기인형。ROP조VEGF-A+405위점C、G등위기인분별위92、106，기인빈솔분별위46.5％，53.5％；대조조VEGF-A+405위점C、G등위기인분별위90、70，기인빈솔분별위56.2％，43.8％；량조비교，차이무통계학의의(X2=3.396，P=0.066)。상관성분석발현，VEGF-A+ 405기인다태성여ROP발생무상관성(OR=0.675，OR95％ CI=0.444，1.026)。ROP조VEGF-A936위점T、C등위기인분별위32、166，기인빈솔분별위16.2％，83.8％；대조조VEGF-A936위점T、C등위기인분별위16、144，기인빈솔분별위1o.o％，90.0％；량조비교，차이무통계학의의(X2 =2.894，P=0.089)。상관성분석발현，VEGF-A936기인다태성여ROP발생무상관성(OR=0.768，OR95％ CI=0.711，0.829)。결론 VEGF-A+ 405화VEGF-A936기인다태성여ROP역감성무관。
ObjectiveTo investigate the correlation between retinopathy of prematurity(ROP)susceptibility and +405G/C and 936T/C polymorphism of vascular endothelial growth factor A(VEGF-A)gene. Methods 99 ROP infants(ROP group)and 80 premature infants(control group)were enrolled in this study. There was no difference of gestational age, birth weight and preoxygenation time between the ROP and control group (P＞0. 05). The peripheral blood was collected, polymorphism genotypes and frequency of VEGF-A+405 and VEGF-A936 were measured by pyrosequencing. Results There are CC, GG, CG genotypes in VEGF-A+405 site, while CC, CT genotypes in VEGF-A 936 site. The VEGF-A+405 gene allele of C, G were 92,106 with the frequencies of 46.5％, 53.5％ in the ROP group, and 90, 70 with the frequencies of 56.2％, 43.8％ in the control group; the difference between two groups was not statistically significant (X2 =3. 396, P=0. 066). There was no correlation between VEGF-A+ 405 polymorphism and ROP susceptibility (OR=0. 675, OR95％ CI=0. 444, 1. 026). The VEGF-A 936 gene allele of C, G were 32,166 with the frequencies of 16.2％, 83.8％ in the ROP group, and 16, 144 with the frequencies of 10.0％, 90.0％ in the control group; the difference between two groups was not statistically significant (X2=2.894, P=0.089). There was no correlation between VEGF-A 936 polymorphism and ROP susceptibility (OR=0. 768, OR95％ CI=0. 711, 0. 829). ConclusionThere is no correlation between VEGF-A+405 or VEGF-A 936 polymorphism and ROP susceptibility.