CAJ | 학술논문

目的检测肿瘤干细胞标志物CD133在原发性肝细胞癌患者癌组织和外周血中的表达,分析CD133+表达与肿瘤进展的关系.方法采集65例肝癌患者癌组织和外周静脉血,免疫组织化学SP法检测癌组织中CD133表达,流式细胞术检测外周血CD45-CD133+细胞百分数,另采集10例正常肝组织和外周血作对照.结果肝癌组织中CD133阳性表达率为6.4%,外周血中CD45-CD133+细胞百分数为2.0%,正常肝组织和外周血中未见CD133阳性表达.差片有统计学意义(P<0.01);CD133阳性细胞含量与门静脉癌栓、肿瘤包膜完整性、病理组织分级、TNM分期显著相关(P值均<0.01).与肿瘤直径、AFP值无相关性(P值均0.05);CD133在癌纽织和外周血中的表达有相关性,二者挈正相关(r=0.36,P<0.01).结论肝癌CD133+细胞数与肿瘤进展密切相关,检测肝癌组织和外周血的CD133表达对肝癌诊断和预后评估有临床实用价值.
목적 검측종류간세포표지물CD133재원발성간세포암환자암조직화외주혈중적표체,분석CD133+표체여종류진전적관계.방법 채집65례간암환자암조직화외주정맥혈,면역조직화학SP법검측암조직중CD133표체,류식세포술검측외주혈CD45-CD133+세포백분수,령채집10례정상간조직화외주혈작대조.결과 간암조직중CD133양성표체솔위6.4%,외주혈중CD45-CD133+세포백분수위2.0%,정상간조직화외주혈중미견CD133양성표체.차편유통계학의의(P<0.01);CD133양성세포함량여문정맥암전、종류포막완정성、병리조직분급、TNM분기현저상관(P치균<0.01).여종류직경、AFP치무상관성(P치균0.05);CD133재암뉴직화외주혈중적표체유상관성,이자설정상관(r=0.36,P<0.01).결론 간암CD133+세포수여종류진전밀절상관,검측간암조직화외주혈적CD133표체대간암진단화예후평고유림상실용개치.
Objective To investigate relationship between CD133 expression and progression of hepatocellular carcinoma (HCC) by detecting expressions of CD133. Methods 65 cases with HCC were enrolled and their HCC tissues and peripheral blood samples were taken. Expression of CD133 in HCC tissue was detected with immunohistochemical method and the percentages of CD45- CD133+ cells in peripheral blood were assayed by the method of flow cytometry (FCM), and 10 healthy persons were chosen as controls. Results In HCC patients, the positive expression rate of CD133 in HCC tissues was 6.4 % and the percentage of CD45- CD133+ cells in peripheral blood was 2.0 %. The expression rate of CD133 was not found in normal liver tissues and peripheral blood (P <0.01). The amount of CD133+ cells was significantly related to portal vein tumor embolus (P<0.01), tumor amicula integrality (P<0.01), pathology grade (P<0.01) and TNM stage (P <0.01), but not related to tumor diameter(P0.05) and AFP value (P0.05). It showed a positive correlation between HCC tissues and peripheral blood (r=0.36, P <0.01). Conclusion The percentage of CD133+ cell in HCC tissues and peripheral blood from HCC patient is greatly correlated to the progression of HCC. It is useful to exam the CD133 expression in predicting the diagnosis and prognosis evaluation.