CAJ | 학술논문

目的对鼻咽癌中染色体11q13上的4个位点进行微卫星多态性分析，明确这些位点染色体等位基因杂合性丢失的情况。方法采用显微切割的方法获取较纯的肿瘤组织，然后用PCR的方法以PYGM、D11S4946 、D11S449和INT-2为引物，对38例鼻咽癌进行微卫星序列分析。结果[ HT5”SS〗 38例鼻咽癌组织中，至少有一个位点出现杂合性缺失者36例，占94.7%。其中D11S4946杂合性缺失的频率最高，占78.8%（26/33），其余的引物分别为：INT-2占5 1.5%(17/33)，PYGM占45.5%（15/33），D11S449占45.7%（16/35）。结论鼻咽癌染色体11q13区发生高频率杂合性缺失，提示缺失区域可能存在与鼻咽癌发生有关的抑癌基因。
목적대비인암중염색체11q13상적4개위점진행미위성다태성분석，명학저사위점염색체등위기인잡합성주실적정황。방법채용현미절할적방법획취교순적종류조직，연후용PCR적방법이PYGM、D11S4946 、D11S449화INT-2위인물，대38례비인암진행미위성서렬분석。결과[ HT5”SS〗 38례비인암조직중，지소유일개위점출현잡합성결실자36례，점94.7%。기중D11S4946잡합성결실적빈솔최고，점78.8%（26/33），기여적인물분별위：INT-2점5 1.5%(17/33)，PYGM점45.5%（15/33），D11S449점45.7%（16/35）。결론비인암염색체11q13구발생고빈솔잡합성결실，제시결실구역가능존재여비인암발생유관적억암기인。
Objective　To analyze the loss o f heterozygosity (LOH) from STS sites at 11q13 in nasopharyngeal carcinoma(NPC). 　Methods　Relatively pure tumor tissues were ob tain ed by microdissetion. PCR was used to perform microsatellite analysis of NPC sam ples using D11S4946, PYGM, D11S449 and INT-2 as primers, which span the M EN-1 gene.　Results　Thirty-six of 38 cases st udied showed LOH in at least one of the loci analyzed. Among the primers used, D11S4946 had the highest frequency of LOH, (78.8%). The LOH frequency was 51.5 % for INT-2, 45.5% for PYGM and 45.7% for D11S449, respectively.　Conclusion　The high frequency of loss of heterozygosity a t 11q13 in NPC implies that there may be some tumor suppressor gene(s) involved in NPC tumorigenesis.