CAJ | 학술논문

目的研究康复功能训练对大鼠脑损伤神经功能恢复及损伤边缘皮质胶原纤维酸性蛋白(GFAP)和离子钙接头分子(Iba-1)表达的影响.方法 SD大鼠131只,按随机数字表法选取30只为假手术组,其余101只制作脑损伤模型.剔除制模未达标大鼠11只,余90只按随机数字表法分为康复训练组、制动组和自由活动组,每组30只大鼠.康复训练组于术后第4天开始每天给予平衡、旋转、行走等功能训练,每项15min,共训练45min,每周6d；制动组置于网状笼内固定；自由活动组和假手术组置于普通笼内饲养.在术后第3天及术后1、2、3和4周对上述4组分别进行神经和运动功能评估,观察其恢复状况；同时采用免疫荧光染色观察损伤区边缘皮质GFAP和Iba-1的表达.结果康复训练组术后2、3和4周在功能评估方面优于制动组和自由活动组(P＜0.05)；术后4周自由活动组较制动组的神经运动功能也有所恢复(P＜0.05).脑损伤术后2、3和4周康复训练组GFAP阳性细胞灰度值和术后3、4周康复训练组Iba-1阳性细胞平均灰度值均明显低于制动组和自由活动组(P＜0.01).结论康复功能训练可促进大鼠脑损伤的神经功能恢复,其机制可能与损伤区边缘皮质星形胶质细胞和小胶质细胞的活化数目减少有关.
목적 연구강복공능훈련대대서뇌손상신경공능회복급손상변연피질효원섬유산성단백(GFAP)화리자개접두분자(Iba-1)표체적영향.방법 SD대서131지,안수궤수자표법선취30지위가수술조,기여101지제작뇌손상모형.척제제모미체표대서11지,여90지안수궤수자표법분위강복훈련조、제동조화자유활동조,매조30지대서.강복훈련조우술후제4천개시매천급여평형、선전、행주등공능훈련,매항15min,공훈련45min,매주6d；제동조치우망상롱내고정；자유활동조화가수술조치우보통롱내사양.재술후제3천급술후1、2、3화4주대상술4조분별진행신경화운동공능평고,관찰기회복상황；동시채용면역형광염색관찰손상구변연피질GFAP화Iba-1적표체.결과 강복훈련조술후2、3화4주재공능평고방면우우제동조화자유활동조(P＜0.05)；술후4주자유활동조교제동조적신경운동공능야유소회복(P＜0.05).뇌손상술후2、3화4주강복훈련조GFAP양성세포회도치화술후3、4주강복훈련조Iba-1양성세포평균회도치균명현저우제동조화자유활동조(P＜0.01).결론 강복공능훈련가촉진대서뇌손상적신경공능회복,기궤제가능여손상구변연피질성형효질세포화소효질세포적활화수목감소유관.
Objective To study the effectsof rehabilitative training on neural function and the expression of glial fibrillary acidic protein (GFAP) and ionized calcium binding adaptor molecule-1 (Iba-1) in rats after traumatic brain injury.Methods A left hemisphere traumatic brain injury model was established in ninety Sprague-Dawley rats.They were then randomly divided into a rehabilitation training group,an immobilization group and a free-running group,with 30 rats in each group.Another thirty rats received sham injury as the shamoperated group.Beginning 4 days post-operation the rats of the rehabilitation training group were given balancing,rotating and walking exercises three times daily,15 min/time,6 d/week.The immobilization group was fixed in mesh cages.The rats of the free-running and sham-operated groups were reared in normal cages without any special training exercise.The rats of all 4 groups were given neural and motor function tests to assess the functional outcome.Immunofluorescence staining was used to evaluate the expressions of GFAP (the marker of astrocytes) and Iba-1 (the marker of microglia) in the cortex close to the iujured region at 3 days,1 week,2 weeks,3 weeks and 4 weeks after injury.Results The average neural and motor function test scores in the rehabilitation training group were significantly better than those in the immobilization and free-running groups at the 2nd week and thereafter.The average scores in the free-running group were significantly better than those in the immobilization group at the 4th week after injury.The immunofluorescence staining showed that the expression of GFAP was lowest in the rehabilitation group at the 2nd week and thereafter.Iba-1 expression was significantly lower only at the 3rd week and beyond.Conclusion Rehabilitative training can improve nerve function recovery in rats after traumatic brain injury,and the functional enhancement may be partially attributed to the downregulation of activated astrocytes and microglia.