中华物理医学与康复杂志
中華物理醫學與康複雜誌
중화물리의학여강복잡지
CHINESE JOURNAL OF PHYSICAL MEDICINE AND REHABILITATION
2009年
9期
604-606
,共3页
局部湿热%微波消融%肿瘤转移
跼部濕熱%微波消融%腫瘤轉移
국부습열%미파소융%종류전이
Local wet hyperthermia%Microwave ablation%Tumor metastasis
目的 研究局部湿热联合微波消融对肿瘤生长和血清干扰素-γ(IFN-γ)、白细胞介素-12(IL-12)的影响,并探讨其意义.方法 48只Balb/c小鼠随机分为荷瘤未处理组、微波消融组、局部湿热组、微波消融+局部湿热组,每组12只.先建立小鼠乳腺癌实验动物模型,14 d后进行微波消融处理,再过7 d后进行局部湿热处理,每周1次,共2次.观察各组肿瘤生长情况、生存情况和肺转移情况,并用酶联免疫吸附法检测血清IFN-γ、IL-12的变化.结果 微波消融+局部湿热组的肿瘤牛长大小和肺转移指数明显低于微波消融组和局部湿热组(P＜0.05),生存时间明显延长(P＜0.05),血清IFN-γ和IL-12的浓度也显著增加(P＜0.05).结论 局部湿热联合微波消融可以较单一热疗方法更有效地抑制肿瘤生长,这可能与其免疫反应的明显增强有关.
目的 研究跼部濕熱聯閤微波消融對腫瘤生長和血清榦擾素-γ(IFN-γ)、白細胞介素-12(IL-12)的影響,併探討其意義.方法 48隻Balb/c小鼠隨機分為荷瘤未處理組、微波消融組、跼部濕熱組、微波消融+跼部濕熱組,每組12隻.先建立小鼠乳腺癌實驗動物模型,14 d後進行微波消融處理,再過7 d後進行跼部濕熱處理,每週1次,共2次.觀察各組腫瘤生長情況、生存情況和肺轉移情況,併用酶聯免疫吸附法檢測血清IFN-γ、IL-12的變化.結果 微波消融+跼部濕熱組的腫瘤牛長大小和肺轉移指數明顯低于微波消融組和跼部濕熱組(P＜0.05),生存時間明顯延長(P＜0.05),血清IFN-γ和IL-12的濃度也顯著增加(P＜0.05).結論 跼部濕熱聯閤微波消融可以較單一熱療方法更有效地抑製腫瘤生長,這可能與其免疫反應的明顯增彊有關.
목적 연구국부습열연합미파소융대종류생장화혈청간우소-γ(IFN-γ)、백세포개소-12(IL-12)적영향,병탐토기의의.방법 48지Balb/c소서수궤분위하류미처리조、미파소융조、국부습열조、미파소융+국부습열조,매조12지.선건립소서유선암실험동물모형,14 d후진행미파소융처리,재과7 d후진행국부습열처리,매주1차,공2차.관찰각조종류생장정황、생존정황화폐전이정황,병용매련면역흡부법검측혈청IFN-γ、IL-12적변화.결과 미파소융+국부습열조적종류우장대소화폐전이지수명현저우미파소융조화국부습열조(P＜0.05),생존시간명현연장(P＜0.05),혈청IFN-γ화IL-12적농도야현저증가(P＜0.05).결론 국부습열연합미파소융가이교단일열료방법경유효지억제종류생장,저가능여기면역반응적명현증강유관.
Objective To investigate the effects of local wet hyperthermia combined with microwave ablationon tumor growth and serum levels of interferon-β(IFN-β)and interleukine-12(IL-12).Methods An experimental mouse model beating mammary carcinoma was established in Balb/c mice and then treated with microwave ablation after 14 d.Seven d after ablation,local wet hyperthermia was administered once weekly for 2 weeks.Tumor growth and lung metastasis,as well as the survival of bearing tumor mice were observed.The serum levels of IFN-β and IL-12 were detected with enzyme-linked immuno sorbent assay(ELISA). Results When local wet hyperthermia was combined with microwave ablation tumor growth and lung metastasis were significantly less than when microwave ablation or local wet hyperthermia was used alone(P＜0.05).Furthermore,the survival of bearing tumor mice in combined therapy group prolonged significantly compared with those receiving either therapy alone(P＜0.05).Serum levels of IFN-β and IL-12 were also significantly higher in combined therapy group compared with the other groups (P＜0.05). Conclusions Local wet hyperthermia combined with microwave ablation is more effective than either local wet hyperthermia or microwave ablation alone in retarding tumor growth,which may be related to the enhanced immune response.
