CAJ | 학술논문

目的建立一种快速、准确、特异件高的方法检测手足口病病原体.方法根据引起手足口病常见致病原肠道病毒71(EV71)、柯萨奇病毒A16(CA16)及肠道病毒(EV),设计相应的引物、探针对35例临床诊断的手足口病患儿及20例正常健康婴儿的粪便进行EV、EV71、CA16三种病原体实时荧光RT-PCR检测,同时对55分标本进行EV71病毒培养分离.结果 35例临床诊断手足口病患儿粪便中EV全部阳性,EV71阳性25例,CA16阳性8例,其中3例为EV71、CA16同时阳性,与临床诊断符合率为85.71%,20例健康体检婴儿粪便中EV病毒5例阳性,EV71、CA16均阴性.结论荧光RT-PCR对手足口病病原体检测准确性高、特异性强,具有快速、廉价等特点,适合于手足口病的早期诊断.
목적 건립일충쾌속、준학、특이건고적방법 검측수족구병병원체.방법 근거인기수족구병상견치병원장도병독71(EV71)、가살기병독A16(CA16)급장도병독(EV),설계상응적인물、탐침대35례림상진단적수족구병환인급20례정상건강영인적분편진행EV、EV71、CA16삼충병원체실시형광RT-PCR검측,동시대55분표본진행EV71병독배양분리.결과 35례림상진단수족구병환인분편중EV전부양성,EV71양성25례,CA16양성8례,기중3례위EV71、CA16동시양성,여림상진단부합솔위85.71%,20례건강체검영인분편중EV병독5례양성,EV71、CA16균음성.결론 형광RT-PCR대수족구병병원체검측준학성고、특이성강,구유쾌속、렴개등특점,괄합우수족구병적조기진단.
Objective To develop a rapid, accurate, specific method to detect causative agent of hand, foot and mouth disease (HFMD). Methods Specific primers and probe were designed based on highly conserved VP1 region of enterovirus 71, coxsackie virus A16 and enterovirus. The sensitivity and specificity of the real-time RT-PCR was evaluated with 35 stool samples collected from pediatric patients with suspected HFMD and 20 clinical samples from health pediatric patients. Results Out of 35 clinical samples from suspected HFMD, 35 samples were identified as positive for enterovirus, 25 clinical samples were identified as positive for enterovirus 71, 8 clinical samples were identified as positive for coxsackie virus A16, among which 3 clinical samples were identified as positive for enterovirus 71 and coxsackie virus A16. The clinical diagnostic accordance rate is 85.71%. Out of 20 clinical samples from normal pediatric patients, 5 clinical samples were identified as positive for enterovirus, 20 clinical samples were negative for enterovirns 71 and coxsackie virus AI6. Conclusion Our results indicate real-time RT-PCR offers a rapid, sensitive, specific and cheap method to detect pathogen of HFMD from clinical specimens.