CAJ | 학술논문

目的探讨Line-1(long interspersed nucleotide element-1)甲基化水平与胃癌临床病理特征的关系.方法收取30例胃癌标本和癌旁正常组织,采用焦磷酸测序技术(Pyrosequencing)对胃癌组织和癌旁正常组织Line-1甲基化水平进行检测.结果 30例胃癌患者胃癌组织中Line-1甲基化平均水平65.8%,而正常组织为75.1%,差异有统计学意义(P＜0.05);30例胃癌组织中,Line-1甲基化水平在不同的发病性别、发病年龄、发生位置和分化程度中差异无统计学意义(P＞0.05);Line-1甲基化程度与浸润深度(T1/T2:68.9%、T3/T4:61.3%)、淋巴结转移(NO:70.0%、N1:69.9%、N2:61.7%、N3:57.6%)和远处转移(MO:67.9%、M1:59.1%)明显相关(P＜0.05),并且随着胃癌的进展而降低.结论 Line-1低甲基化与胃癌浸润和转移有关,可能参与胃癌的进展.
목적 탐토Line-1(long interspersed nucleotide element-1)갑기화수평여위암림상병리특정적관계.방법 수취30례위암표본화암방정상조직,채용초린산측서기술(Pyrosequencing)대위암조직화암방정상조직Line-1갑기화수평진행검측.결과 30례위암환자위암조직중Line-1갑기화평균수평65.8%,이정상조직위75.1%,차이유통계학의의(P＜0.05);30례위암조직중,Line-1갑기화수평재불동적발병성별、발병년령、발생위치화분화정도중차이무통계학의의(P＞0.05);Line-1갑기화정도여침윤심도(T1/T2:68.9%、T3/T4:61.3%)、림파결전이(NO:70.0%、N1:69.9%、N2:61.7%、N3:57.6%)화원처전이(MO:67.9%、M1:59.1%)명현상관(P＜0.05),병차수착위암적진전이강저.결론 Line-1저갑기화여위암침윤화전이유관,가능삼여위암적진전.
Objective To elucidate the correlation of methylation of long interspersed nucleotide element-1 (Line-1) with clinicalopathological features of gastric cancer.Methods Gastric cancer tissues and paracancerous tissues were collected from 30 patients and methylation status of Line-1 was detected in caner and corresponding normal tissues by using Pyrosequencing.Results The methylation level of Line-1in normal gastric and gastric cancer tissues was 75.1% and 65.8% respectively (P＜0.05).Among 30cancer tissues,methylation status of Line-1 was closely related to tumor infiltration (T1/T2:68.9%,T3/T4:61.3% ),lymph nodes metastasis ( NO:70.0%,N1:69.9%,N2:61.7%,N3:57.6% ) and distant metastasis ( MO:67.9%,M1:59.1% ) ( P ＜0.05) and declined with tumor progression,but wasn't associated with age,gender,location and differentiation ( P＞ 0.05 ).Conclusion Hypomethylation of Line-1 was remarkably related to infiltration and metastasis of gastric cancer and may contribute to the tumor progression.