CAJ | 학술논문

目的观察超短波对兔激素性股骨头缺血性坏死骨组织中骨形态形成蛋白2表达的影响.方法 30只新西兰大白兔分为正常组(4只)和干预组(26只),干预组采用马血清联合激素复制兔激素性股骨头坏死的模型,经x线验证造模成功的有20只,再随机分为超短波组(10只)和造模组(10只).造模组不予任何干预;超短波组给予超短波治疗,第一个疗程15 d,无热量;第二个疗程15 d,微热量,治疗间隔5 d.空气栓塞快速处死动物,并取兔双侧股骨头,应用实时荧光定量PCR检测骨组织中BMP-2mRNA的基因表达情况.结果造模结束时,干预组BMP-2的表达为正常组的0.2001倍(P＜0.01),差异有统计学意义;第一疗程结束时,超短波组BMP-2mRNA表达量为造模组的2.738倍,第二疗程结束时,超短波组BMP-2mRNA表达量为造模组的10.1260倍(均P＜0.01),差异具有统计学意义.结论超短波可以提高激素性股骨头坏死骨组织BMP-2mRNA的表达,从而促进缺血性坏死股骨头的修复.
목적 관찰초단파대토격소성고골두결혈성배사골조직중골형태형성단백2표체적영향.방법 30지신서란대백토분위정상조(4지)화간예조(26지),간예조채용마혈청연합격소복제토격소성고골두배사적모형,경x선험증조모성공적유20지,재수궤분위초단파조(10지)화조모조(10지).조모조불여임하간예;초단파조급여초단파치료,제일개료정15 d,무열량;제이개료정15 d,미열량,치료간격5 d.공기전새쾌속처사동물,병취토쌍측고골두,응용실시형광정량PCR검측골조직중BMP-2mRNA적기인표체정황.결과 조모결속시,간예조BMP-2적표체위정상조적0.2001배(P＜0.01),차이유통계학의의;제일료정결속시,초단파조BMP-2mRNA표체량위조모조적2.738배,제이료정결속시,초단파조BMP-2mRNA표체량위조모조적10.1260배(균P＜0.01),차이구유통계학의의.결론 초단파가이제고격소성고골두배사골조직BMP-2mRNA적표체,종이촉진결혈성배사고골두적수복.
Objective To observe the effect of ultrashortwave therapy on the expression of BMP-2 in steroid-induced avascular necrosis of the femoral head(SANFH). Methods Thirty New-Zealand white rabbits were randomly divided into two groups:a normal group(4 rabbits)and an intervention group(26 rabbits).An animal model of steroid-induced avascular necrosis of the femoral head was induced through the intravenous injection of horse blood serum and the intraperitoneal injection of methylprednisolone in the intervention group.X-ray examination confirmed the successful induction of SANFH in 20 of the rabbits,who were then subdivided at random into an uhrashortwave therapy group(1 0 rabbits)and a model group(1 0 rabbits).The rabbits in the ultrashortwave group were treated with ultrashortwave therapy in two 1 5-day courses,with a 5-day interval between them. Non-thermal ultrashortwave therapy was given in the first course,then mild thermal uhrashortwave therapy was given in the second.Those in the model group were reared without ultrashortwave therapy.All the animals were sacrificed immediately after the end of the last treatment,and their femoral heads were sampled to observe the expression of BMP-2 using real time fluorescent quantitative PCR. Results The expression of BMP-2 mRNA in the intervention group was only one fifth of that observed in the normal group.It was 2.7 to 10 times that observed in the model group.Both differences were significant at the 1% confidence level. Conclusion Ultrashortwave therapy can promote the expression of BMP-2 and improve reactivation in avascular necrosis of the femoral head.