CAJ | 학술논문

目的探讨急性淋巴细胞白血病（ALL）和慢性髓性白血病（CML）易感性与HLA-DRB1基因多态性之间的关联性，找出急性淋巴细胞白血病和慢性髓性白血病的易感基因。方法采用序列特异性引物聚合酶链反应（PCR-SSP）DNA分型技术对56例ALL患者、48例CML患者和105例健康对照进行了HLA-DRB1基因分型。结果 ALL患者组与HLA-DR7基因关联,基因频率为24.1%，RR =2.56，χ2=7.34，P<0.01；CML患者组与HLA-DR4基因关联，基因频率为22.9%，RR =5.076，χ2=17.88，P<0.01；其他等位基因频率在实验组与对照组间差异无显著性。结论提示在河南汉族人群中，HLA-DR7与ALL有关联，HLA-DR4与CML有关联。
목적탐토급성림파세포백혈병（ALL）화만성수성백혈병（CML）역감성여HLA-DRB1기인다태성지간적관련성，조출급성림파세포백혈병화만성수성백혈병적역감기인。방법채용서렬특이성인물취합매련반응（PCR-SSP）DNA분형기술대56례ALL환자、48례CML환자화105례건강대조진행료HLA-DRB1기인분형。결과 ALL환자조여HLA-DR7기인관련,기인빈솔위24.1%，RR =2.56，χ2=7.34，P<0.01；CML환자조여HLA-DR4기인관련，기인빈솔위22.9%，RR =5.076，χ2=17.88，P<0.01；기타등위기인빈솔재실험조여대조조간차이무현저성。결론제시재하남한족인군중，HLA-DR7여ALL유관련，HLA-DR4여CML유관련。
Objective　To explore the possible association between HLA-DRB1 allele polymorphism and susceptibility to acute lymphoblastic leukemia(ALL) as well as chronic myelogenous leukemia(CML) in Chinese He′nan Han People and to find the predisposing genes of ALL and CML. Methods　105 normal people and 56 ALL patients and 48 CML patients of He′nan Han nationality were tested for HLA-DRB1 by polymerase chain reaction with sequence specific primers(PCR-SSP). Results　Compared with normal controls, the frequency of HLA-DR7 was significantly increased in ALL group with a relative risk (RR) as 2.56 (χ2 =7.34, P<0.01) and with an etiological fraction (EF) as 0.29; the frequencies of other DRB1 alleles were not significantly increased in the ALL group. The frequency of HLA-DR4 was significantly increased in CML patient group, with a relative risk (RR) as 5.076 (χ2=17.88, P<0.01) and with an etiological fraction (EF) as 0.18, while the frequencies of other DRB1 alleles were not significantly increased in CML group. Conclusion　These results showed that susceptibility to ALL is positively related to HLA-DR7 gene and susceptibility to CML is strongly correlated with HLA-DR4 gene.