CAJ | 학술논문

目的建立单纯局灶性脑内血肿动物模型。方法利用血液自凝特性，运用双套管、分次注血法，结合微量泵和立体定向术，以不同注血量将新鲜未肝素化自体血注入34只兔脑基底节外侧区。通过连续冰冻切片计算血肿体积，并观察血肿本身及周围组织形态。结果血肿位于基底外侧区、皮层下组织内侧和内囊前外方，85.29%(29/34)为单纯局灶性脑内血肿；脑内血肿主要由血细胞组成，其体积大小因注血量和血肿形成时间的不同而异(P＜0.05)；血肿周围组织在注血后4h就有水肿表现。结论本实验使自体血注入法脑内血肿模型达到稳定、可控，便于进一步定量研究。
목적건립단순국조성뇌내혈종동물모형。방법이용혈액자응특성，운용쌍투관、분차주혈법，결합미량빙화입체정향술，이불동주혈량장신선미간소화자체혈주입34지토뇌기저절외측구。통과련속빙동절편계산혈종체적，병관찰혈종본신급주위조직형태。결과혈종위우기저외측구、피층하조직내측화내낭전외방，85.29%(29/34)위단순국조성뇌내혈종；뇌내혈종주요유혈세포조성，기체적대소인주혈량화혈종형성시간적불동이이(P＜0.05)；혈종주위조직재주혈후4h취유수종표현。결론본실험사자체혈주입법뇌내혈종모형체도은정、가공，편우진일보정량연구。
Objective　To develop a simple local intracerebral hematoma in 34 rabbits. Methods　After stereotactically placing a cannula into the outboard area of ganglia, different volume of fresh unclotted blood was injected in with a micro pump. The intracerebral hematoma was developed by the repetitious injection method, in which the first clot could block the actual hematoma back along the needle track. The metamorphosis of hematoma and perihematoma histological changes was observed and the hematoma volume was measured on serial freezing sections. Results　Hematoma was located in outboard area of ganglia, inner area of cerebral cortex and front lateral area of internal capsule. 85.29 %(29/34) intracerebral hematoma was established without rupturing into ventricular system, subarachnoid or subdural space. The hematoma was made of red blood cells and its size was varied with the injected blood volume and the time for hematoma formation (P<0.05). The perihematoma edema could be found in white mater 4 h after blood injection. Conclusion　In this experiment, the intracerebral hematoma model in rabbits was stable and reproducible. This model of intracerebral hemorrhage fits for further quantitative investigation.