CAJ | 학술논문

目的了解广州地区肝炎患者中庚型肝炎病毒(HGV)的感染情况及基因型特点。方法采用逆转录-套式聚合酶链反应(RT-nested PCR)方法检测肝炎患者血清中HGV RNA，引物位于HGV基因组的非编码区，并对扩增产物进行直接测序。结果 251例急慢性肝炎血清中共检出25例HGV RNA阳性，总阳性率为9.96％，其中56例非甲～戊型肝炎中检出4例阳性(7.14％)，77例乙型肝炎(HB)中检出8例阳性(10.39％)，118例丙型肝炎(HC)中检出13例阳性(11.17％)；2株HGV广州株核苷酸之间的同源性为98.0％，与非洲株的同源性分别为81.7％和84.2％，与美国株的同源性均为91.1％。结论广州地区肝炎患者中存在HGV感染，2株HGV广州株可能为同一基因型，且与HGV美国株具有较高的同源性。
목적료해엄주지구간염환자중경형간염병독(HGV)적감염정황급기인형특점。방법채용역전록-투식취합매련반응(RT-nested PCR)방법검측간염환자혈청중HGV RNA，인물위우HGV기인조적비편마구，병대확증산물진행직접측서。결과 251례급만성간염혈청중공검출25례HGV RNA양성，총양성솔위9.96％，기중56례비갑～무형간염중검출4례양성(7.14％)，77례을형간염(HB)중검출8례양성(10.39％)，118례병형간염(HC)중검출13례양성(11.17％)；2주HGV엄주주핵감산지간적동원성위98.0％，여비주주적동원성분별위81.7％화84.2％，여미국주적동원성균위91.1％。결론엄주지구간염환자중존재HGV감염，2주HGV엄주주가능위동일기인형，차여HGV미국주구유교고적동원성。
Objective To ifivestigate hepatitis G virus (HGV) infection in hepatitis patients and the gene type of HGV in Guangzhou. Methods Reverse transcriptase-nested polymerase chain reaction (RTnested PCR) method using primers locating in noncoding region of HGV genome was used for detecting HGV RNA in sera of hepatitis patients and the amplified products were directly sequenced. Results 25 cases were HGV RNA positive in 251 sera of acute and chronic hepatitis(9.96% ) ,of which 4 cases were positive in 56 sera with hepatitis non A-E(7.14%),8 cases were positive in 77 sera with hepatitis B ( 10.39 % ), 13 cases were positive in 118 sera with hepatitis C ( 11.17 % ). The identity of nucleotide sequence was 98.0 % between 2 Guangzhou HGV isolates, both were 91.1% with American isolate, 81.7 % and 84.2 % respectively between 2 Guangzhou isolates and African isolate. Conclusion HGV infection exists in hepatitis patients, 2 Guangzhou HGV isolates may belong to the same gene type and both of them have a high homology with American HGV isolate.