CAJ | 학술논문

目的研究小鼠自体肝脏星状细胞联合同种异体胰岛细胞移植的新方法对胰岛移植物存活时间的作用.方法选择雄性BALB/c小鼠为胰岛移植模型的供者,雄性C57BL/6糖尿病小鼠为受者.随机将受者分为A、B两组.A组:仅采用供者的胰岛细胞移植;B组:采用受者的肝脏星状细胞(HSCs)与供者胰岛细胞混合后共同移植.术后定期测定受者尾静脉血的血糖含量.结果 B组受者胰岛移植物的存活时间明显延长,血糖含量维持正常的中位时间为66 d(30～180 d),而A组血糖含量维持正常的中位时间为11 d(9～15 d),两组比较,差异有统计学意义(P＜0.001).结论受者肝脏星状细胞能延长共同移植的同种异体胰岛移植物存活时间.
목적 연구소서자체간장성상세포연합동충이체이도세포이식적신방법대이도이식물존활시간적작용.방법 선택웅성BALB/c소서위이도이식모형적공자,웅성C57BL/6당뇨병소서위수자.수궤장수자분위A、B량조.A조:부채용공자적이도세포이식;B조:채용수자적간장성상세포(HSCs)여공자이도세포혼합후공동이식.술후정기측정수자미정맥혈적혈당함량.결과 B조수자이도이식물적존활시간명현연장,혈당함량유지정상적중위시간위66 d(30～180 d),이A조혈당함량유지정상적중위시간위11 d(9～15 d),량조비교,차이유통계학의의(P＜0.001).결론 수자간장성상세포능연장공동이식적동충이체이도이식물존활시간.
Objective To study the prolongation of allo-islet grafts by co-transplanted hepatic stellate cells(HSCs)in mice.Methods Allo-islet transplant modelin mice were set up in two groups:group A.only islet transplanted group;group B,allo-islet co-transplanted with HSCs group.In group B,HSCs isolated from C5781/6 mice were cultured in vitro and co-transplanted with BALB/cmice islet under C5781/6 renal capsule.The recipients were streptozotocin(STZ)-indtmed diabetes C5781/6 mice.The post graft survival time was monitored by blood glucose.Results In this newly established model.we found that the co-transplanted group had a median graft survival time of 66 days (from 30 to 180 days,n=8),while the only islet transplanted group had a median survival time of 11 days(from 9 tO 15 days,n=10)(P＜0.001).Conclusion Our findings in the new islet transplant model confirm that co-transplanted recipient HSCs can induce allo-islet graft long-term survival in mice without immunosuppression.