CAJ | 학술논문

目的通过对云南非铀矿山矿工血清IL-2、IL-6和TNF-α浓度及基因多态性位点的检测,对氡致非铀矿山矿工机体生物效应进行初步探讨.方法血清中IL-2、IL-6和TNF-α的浓度测定采用双抗夹心ABC-ELISA法.TNF-α(-308,G→A)检测基因型采用基因体外扩增限制性片段长度多态性分析方法 ,IL-2(-330,T→G)基因型检测采用PCR体外扩增后测序的方法 .结果矿工组与对照组比较,年龄(F=1.07,P>0.05),工龄(F=0.40,P>0.05),血清IL-2浓度(F=0.71,P>0.05),血清IL-6浓度(F=1.09,P>0.05),血清TNF-α浓度(F=0.95,P>0.05),IL-2基因型频度(X2=0.02,P>0.05)和TNF-α基因型频度(X2=2.21,P>0.05),差异均无统计学意义.结论云南东川非铀矿山矿工血清IL-2、IL-6和TNF-α平均浓度有下降的趋势,TNF-α(-308,A/A)基因型频度有增高的趋势.
목적 통과대운남비유광산광공혈청IL-2、IL-6화TNF-α농도급기인다태성위점적검측,대동치비유광산광공궤체생물효응진행초보탐토.방법 혈청중IL-2、IL-6화TNF-α적농도측정채용쌍항협심ABC-ELISA법.TNF-α(-308,G→A)검측기인형채용기인체외확증한제성편단장도다태성분석방법 ,IL-2(-330,T→G)기인형검측채용PCR체외확증후측서적방법 .결과 광공조여대조조비교,년령(F=1.07,P>0.05),공령(F=0.40,P>0.05),혈청IL-2농도(F=0.71,P>0.05),혈청IL-6농도(F=1.09,P>0.05),혈청TNF-α농도(F=0.95,P>0.05),IL-2기인형빈도(X2=0.02,P>0.05)화TNF-α기인형빈도(X2=2.21,P>0.05),차이균무통계학의의.결론 운남동천비유광산광공혈청IL-2、IL-6화TNF-α평균농도유하강적추세,TNF-α(-308,A/A)기인형빈도유증고적추세.
Objective To study biologic effect of high radon exposure in non-uranium miners by measurements of serum levels of 1L-2, IL-6 and TNF-u and study on their gene polymorphism. Methods Serum levels of IL-2, IL-6 and TNF-αin the miners selected from a Yunnan-based copper mine were measured by ABC-ELISA. TNF-α (-308,G→A) genotypes were identified by RFLP-PCR, IL-2 (-330, T→G) genotypes by sequence analysis. Results Compared the miners with its control group, there were no statistical significance of the concentrations of serum IL-2 (F=0.71, P>0.05), IL-6 (F=1.09, P>0.05) and TNF-α(F=0.95,P>0.05). Frequencies of IL-2 (-330, T→G) genotypes (X2=0.02, P>0.05) and TNF-α(-308, G→A) genotype (x2= 2.21, P>0.05) were shown no statistical significance too. Conclusions Compared with the control group, concentrations of serum IL-2, IL-6 and TNF-n in miners working in the copper mine was lower, frequencies of genotypes of TNF-o (-308,A/A) was higher in the miners. But all the differences were not statistical significant.