中华儿科杂志
中華兒科雜誌
중화인과잡지
Chinese Journal of Pediatrics
2012年
2期
136-140
,共5页
动脉导管%一氧化氮%硫化氢%胱硫醚-γ-裂解酶
動脈導管%一氧化氮%硫化氫%胱硫醚-γ-裂解酶
동맥도관%일양화담%류화경%광류미-γ-렬해매
Ductus arteriosus%Nitric oxide%Hydrogen sulfide%Cystathionine-γ-lyase
目的 探讨一氧化氮(NO)供体硝普钠对早产兔动脉导管胱硫醚-γ-裂解酶(CSE)-硫化氢(H2S)体系的影响.方法 孕龄21 d日本大耳白兔16只,随机分为对照组,腹腔注射硝普钠(NO供体)小剂量(1 mg/kg)组,中剂量(2.5 mg/kg)组,大剂量(5 mg/kg)组,每组4只,对照组单纯用空针穿刺孕兔腹腔,各腹腔注射硝普钠组待孕龄23和25 d时分别腹腔注射相应剂量的硝普钠.待孕龄第26天时对各组动物进行麻醉解剖取胎兔(对照组取得胎兔28只,硝普钠小剂量组27只,中剂量组29只,大剂量组26只),胎兔心脏取血1ml后立即解剖胎兔取动脉导管组织.用去蛋白方法测定胎兔血浆H2S含量,用实时荧光定量RT-PCR测定动脉导管组织中CSE基因,用Western-blot测CSE蛋白表达情况.结果 对照组胎兔血浆H2S含量为(55.68±6.57) μmol/L,动脉导管组织CSEmRNA表达量为1.07±4.12；硝普钠腹腔注射小剂量组分别为(60.02±6.09) μmol/L,3.46±0.18；中剂量组为(64.71 ±7.12) μmol/L,10.93 ±0.22；大剂量组为(70.63±8.07) μmol/L和19.57 ±0.17,小剂量组与对照组、中剂量组与小剂量组、大剂量组与中剂量组比较均P＜ 0.05,胎兔动脉导管CSE蛋白表达随着孕兔注射硝普钠剂量的增大而逐渐增多.结论 硝普钠对早产兔动脉导管中CSE-H2S体系在一定浓度范围内具有浓度依赖性促进作用.
目的 探討一氧化氮(NO)供體硝普鈉對早產兔動脈導管胱硫醚-γ-裂解酶(CSE)-硫化氫(H2S)體繫的影響.方法 孕齡21 d日本大耳白兔16隻,隨機分為對照組,腹腔註射硝普鈉(NO供體)小劑量(1 mg/kg)組,中劑量(2.5 mg/kg)組,大劑量(5 mg/kg)組,每組4隻,對照組單純用空針穿刺孕兔腹腔,各腹腔註射硝普鈉組待孕齡23和25 d時分彆腹腔註射相應劑量的硝普鈉.待孕齡第26天時對各組動物進行痳醉解剖取胎兔(對照組取得胎兔28隻,硝普鈉小劑量組27隻,中劑量組29隻,大劑量組26隻),胎兔心髒取血1ml後立即解剖胎兔取動脈導管組織.用去蛋白方法測定胎兔血漿H2S含量,用實時熒光定量RT-PCR測定動脈導管組織中CSE基因,用Western-blot測CSE蛋白錶達情況.結果 對照組胎兔血漿H2S含量為(55.68±6.57) μmol/L,動脈導管組織CSEmRNA錶達量為1.07±4.12；硝普鈉腹腔註射小劑量組分彆為(60.02±6.09) μmol/L,3.46±0.18；中劑量組為(64.71 ±7.12) μmol/L,10.93 ±0.22；大劑量組為(70.63±8.07) μmol/L和19.57 ±0.17,小劑量組與對照組、中劑量組與小劑量組、大劑量組與中劑量組比較均P＜ 0.05,胎兔動脈導管CSE蛋白錶達隨著孕兔註射硝普鈉劑量的增大而逐漸增多.結論 硝普鈉對早產兔動脈導管中CSE-H2S體繫在一定濃度範圍內具有濃度依賴性促進作用.
목적 탐토일양화담(NO)공체초보납대조산토동맥도관광류미-γ-렬해매(CSE)-류화경(H2S)체계적영향.방법 잉령21 d일본대이백토16지,수궤분위대조조,복강주사초보납(NO공체)소제량(1 mg/kg)조,중제량(2.5 mg/kg)조,대제량(5 mg/kg)조,매조4지,대조조단순용공침천자잉토복강,각복강주사초보납조대잉령23화25 d시분별복강주사상응제량적초보납.대잉령제26천시대각조동물진행마취해부취태토(대조조취득태토28지,초보납소제량조27지,중제량조29지,대제량조26지),태토심장취혈1ml후립즉해부태토취동맥도관조직.용거단백방법측정태토혈장H2S함량,용실시형광정량RT-PCR측정동맥도관조직중CSE기인,용Western-blot측CSE단백표체정황.결과 대조조태토혈장H2S함량위(55.68±6.57) μmol/L,동맥도관조직CSEmRNA표체량위1.07±4.12；초보납복강주사소제량조분별위(60.02±6.09) μmol/L,3.46±0.18；중제량조위(64.71 ±7.12) μmol/L,10.93 ±0.22；대제량조위(70.63±8.07) μmol/L화19.57 ±0.17,소제량조여대조조、중제량조여소제량조、대제량조여중제량조비교균P＜ 0.05,태토동맥도관CSE단백표체수착잉토주사초보납제량적증대이축점증다.결론 초보납대조산토동맥도관중CSE-H2S체계재일정농도범위내구유농도의뢰성촉진작용.
Objective To investigate the impact of sodium nitroprusside (a nitric oxide donor) in the ductus arteriosus in preterm rabbits on hydrogen sulfide (H2S)-cystathionine-γ-lyase (CSE) system.Methods For 16 Japanese white rabbits pregnant for 21 days were randomly divided into four groups,each of the following groups had 4 rabbits: control group,intraperitoneal injection of sodium nitroprusside 1 mg/kg,2.5 mg/kg,and 5.0 mg/kg groups.The rabbits in control group had a peritoneal puncture with a simple hollow needle,and those in the other groups were given corresponding dose of intraperitoneal injection of sodium nitroprusside at gestational age 23 and 25 days,respectively.At gestational age 26 days the fetuses of the pregnant rabbits were removed surgically,and 28 fetal rabbits were obtained from the control group,27 from the sodium nitroprusside small dose group,29 from the midium dose group,and 26 from the large dose group.The fetal heart blood sample of 1 ml was taken from each fetus,and immediately after sampling the arterial ductal tissues were dissected. Fetal rabbit plasma proteins hydrogen sulfide content was determined by using de-protein method,and real time quantitative RT-PCR was used for determination of arterial tissue CSE gene and western-blotting was used for measuring protein expression of CSE.Results In control group hydrogen sulfide content of fetal rabbits plasma (55.68 ± 6.57 ) μmol/L and arterial tissue CSE mRNA expression was 1.07 ± 4.12 ; the parameters in intraperitoneal injection of sodium nitroprusside group 1 mg/kg were (60.02 ± 6.09) μmol/L and 3.46 ± 0.18; in intraperitoneal injectionof sodium nitroprusside group 2.5 mg/kg,were (64.71 ±7.12) μmol/L and 10.95 ±0.22; and in intraperitoneal injection of sodium nitroprusside group 1 mg/kg were ( 70.63 ± 8.07 ) μ mol/L and 19.56 ± 0.17.Comparison between small dose group and control group,midium dose group and small dose group,high dose group and midium dose group showed that the above data were significantly different P ＜ 0.05,with the injection of sodium nitroprusside CSE protein expression increased gradually with increasing doses.Conclusion Sodium nitroprusside showed an enhancing effect on preterm CSE-H2 S system in rabbit ductus arteriosus in a certain range of concentration in a dose-dependent manner.
