CAJ | 학술논문

采用免疫组织化学方法检测小儿常见恶性肿瘤突变型P_(53)蛋白的表达情况并探讨其临床意义.甩DO-1P_(53)单克隆抗体,LSAB法测定45例小儿恶性肿瘤石蜡切片的P_(53)表达.19例神经母细胞瘤3例阳性,均为Ⅳ期患儿,其中1例阳性为睾丸转移瘤而原发瘤阴性;16例肾母细胞瘤4例阳性,其中3例预后不良型均为阳性;10例恶性淋巴瘤5例阳性,均为Ⅰ、Ⅳ期患儿.结果表明突变型P_(53)蛋白的表达与三种肿瘤的临床分期、组织学类型有关,可能为三种肿瘤转移、复发的原因,为小儿恶性肿瘤预后不良的一个重要指标.
채용면역조직화학방법검측소인상견악성종류돌변형P_(53)단백적표체정황병탐토기림상의의.솔DO-1P_(53)단극륭항체,LSAB법측정45례소인악성종류석사절편적P_(53)표체.19례신경모세포류3례양성,균위Ⅳ기환인,기중1례양성위고환전이류이원발류음성;16례신모세포류4례양성,기중3례예후불량형균위양성;10례악성림파류5례양성,균위Ⅰ、Ⅳ기환인.결과표명돌변형P_(53)단백적표체여삼충종류적림상분기、조직학류형유관,가능위삼충종류전이、복발적원인,위소인악성종류예후불량적일개중요지표.
Objective:To detect the expression of mutant P53 in Children's malignant tumors by immunohistochemical method and to discuss its clinical significance.Method:45 polysine-coated slides were stained with DO-1 P_(53) monoclonal antibodies by the method of LSAB.Results:There were 3 cases(stage Ⅳ)of positive mutant P_(53) in 19 neuroblastomas.Of them,one case of testicular metastatic tumor was positive,but the primary tumor was negative.Four cases(UF type in 3)of the 16 nephroblastomas were positive.Five of the 10 malignant lymphomas were positive.All of them belonged to stage Ⅲ orⅣ.Conclusion:The expression of mutant P_(53) is related to clinical stage and histologic type of the above three tumors.It is probably a cause of tumor metastasis and relapse.The mutant P_(53) being positive appears to be an important prognostic factor of some tumors in children.