CAJ | 학술논문

目的评价七氟烷预处理对脂多糖(lipopolysaccharide,LPS)所致大鼠急性肺损伤(acute lung injury,ALI)的影响.方法 72只SD大鼠随机分成6组:NS组、LPS组和七氟烷预处理(S-1 h组、S-6 h组、S-12 h组、S-24 h组).通过气管内滴注LPS建立大鼠ALI模型.大鼠在气道内给予LPS或NS后6 h处死,检测不同时间点七氟烷预处理(2.4%,30 min)对支气管肺泡灌洗液(bronchoalveolar lavage fluid,BALF)中白细胞计数、细胞因子TNF-α和IL-1β水平,肺组织髓过氧化物酶(myeloperoxidase,MPO)活性,肺血管通透性及肺组织病理学等的影响.结果与NS组相比,LPS组肺组织损伤程度,BALF白细胞计数以及TNF-α和IL-1β水平,血管通透性和肺组织MPO活性均显著增高(P<0.01).给予LPS前1 h和24 h七氟烷预处理能降低肺组织MPO活性,BALF中IL-1β水平及白细胞计数(P<0.01),而S-6h组和S-12 h组与IPS组相比无明显差别.七氟烷预处理均能够降低肺血管通透性和BALF中TNF-α水平(P<0.01),且以S-1 h和S-24h组为显著(P<0.01).提示七氟烷预处理对LPS所致肺损伤具有早期和延时的保护作用.结论气道内给予LPS1 h和24 h前七氟烷预处理对LPS致大鼠ALI具有保护作用.
목적 평개칠불완예처리대지다당(lipopolysaccharide,LPS)소치대서급성폐손상(acute lung injury,ALI)적영향.방법 72지SD대서수궤분성6조:NS조、LPS조화칠불완예처리(S-1 h조、S-6 h조、S-12 h조、S-24 h조).통과기관내적주LPS건립대서ALI모형.대서재기도내급여LPS혹NS후6 h처사,검측불동시간점칠불완예처리(2.4%,30 min)대지기관폐포관세액(bronchoalveolar lavage fluid,BALF)중백세포계수、세포인자TNF-α화IL-1β수평,폐조직수과양화물매(myeloperoxidase,MPO)활성,폐혈관통투성급폐조직병이학등적영향.결과 여NS조상비,LPS조폐조직손상정도,BALF백세포계수이급TNF-α화IL-1β수평,혈관통투성화폐조직MPO활성균현저증고(P<0.01).급여LPS전1 h화24 h칠불완예처리능강저폐조직MPO활성,BALF중IL-1β수평급백세포계수(P<0.01),이S-6h조화S-12 h조여IPS조상비무명현차별.칠불완예처리균능구강저폐혈관통투성화BALF중TNF-α수평(P<0.01),차이S-1 h화S-24h조위현저(P<0.01).제시칠불완예처리대LPS소치폐손상구유조기화연시적보호작용.결론 기도내급여LPS1 h화24 h전칠불완예처리대LPS치대서ALI구유보호작용.
Objective To investigate the effects of sevoflurane pretreatment on Lipopolysaccharide (LPS)-induced acute lung injury (ALI) in rats. Methods Seventy -two Sprague -Dawley rats were randomly divided into 6 groups: NS group, LPS group and sevoflurane pretreatment (S-l h group, S-6 h group, S-12 h group and S-24 h group). The rat model of ALI was established by intratracheal instillation of LPS. Animals were sacrificed at 6 h after LPS or NS administration. Leukocyte count, concentration of TNF-α and IL-β in bronchoalveolar lavage fluid (BALF); pulmonary capillary permeability, myeloperoxidase (MPO) activity of lung tissue; lung histological changes were compared in rats with or without sevoflurane pretreatment (2.4% inspired for 30 min) at different times before LPS instillation. Results Compared with the NS group,severe injury of lung tissues and increase in leukocyte count in BALF, Production of TNF-α and IL-1β in BALF, pulmonary capillary permeability and MPO activity in the lung were significantly increased in rats treated with LPS (P<0.01). MPO activity, leukocyte count and production of IL-1βin BALF were reduced when sevoflurane was given 1 or 24 h before but not at 6 or 12 h before LPS instillation(P<0.01). Sevoflurane pretreatment also attenuated pulmonary capillary permeability and production of TNF-α in BALF (P<0.01). Pulmonary capillary permeability and concentration of TNF-α in S-l h and S-24 h group was lower than S-6 h and S-12 h group (P<0.01). Sevoflurane pretreatment was effective at 1 h and 24 h suggesting sevoflurane has early and late protection against LPS-induced lung injury. Conclusion Sevoflurane pretreatment has protective effects against acute lung injury when given 1 or 12 h before LPS instillation.