CAJ | 학술논문

目的观察SOD、MDA在骨髓间充质干细胞(marrow mesenchymal stem cells,MSCs)修复皮肤光老化表达.方法采用密度梯度离心法分离培养MSCs,利用UVB灯管照射豚鼠制备皮肤光老化模型,随机分为正常组、模型组及治疗组.检测皮肤组织中SOD、MDA表达.结果 MSCs细胞在豚鼠皮肤照射区局部多点皮内注射后,MDA表达较模型组明显下降(P＜0.01),但高于正常组.治疗组中SOD表达明显高于模型组(P＜0.01),低于正常组.结论 MSCs注射可以清除体内的氧自由基等有害物质,从而使抗氧化平衡系统保持稳定,MSCs对皮肤光老化有治疗作用.
목적 관찰SOD、MDA재골수간충질간세포(marrow mesenchymal stem cells,MSCs)수복피부광노화표체.방법 채용밀도제도리심법분리배양MSCs,이용UVB등관조사돈서제비피부광노화모형,수궤분위정상조、모형조급치료조.검측피부조직중SOD、MDA표체.결과 MSCs세포재돈서피부조사구국부다점피내주사후,MDA표체교모형조명현하강(P＜0.01),단고우정상조.치료조중SOD표체명현고우모형조(P＜0.01),저우정상조.결론 MSCs주사가이청제체내적양자유기등유해물질,종이사항양화평형계통보지은정,MSCs대피부광노화유치료작용.
Objective To observe the expressions of SOD, MDA on photoaging skin when it was repaired by bone marrow mesenchymal stem cells (MSCs) . Methods MSCs were isolated and cultured according to density gradient centrifugation. The model of photoaging skin was prepared in guinea pigs by exposure to UVB lamp. The animals were divided into the normal group, model group and treatment group. The expressions of SOD and MDA were detected in skin tissue. Results The expression of MDA in treatment group was significantly decreased compared with that of the model ( P ＜ 0. 01 ), but higher than that in the normal group. The expression of SOD in treatment group was significantly higher than that of model group ( P ＜ 0. 01 ), but lower than that of normal group. Conclusions MSCs can remove the harmful substances such as oxygen free radicals and keep the antioxidant balance of the system. MSCs play the role on the treatment of skin photoaging.