CAJ | 학술논문

目的探讨寻常型银屑病与人类白细胞抗原(HLA)复合体C基因座位第73位氨基酸密码子的相关性。方法采用聚合酶链反应—变性聚丙烯酰胺凝胶电泳—DNA银染、测序，检测了104例寻常型银屑病患者和110例健康对照者的HLA-C基因第73位氨基酸密码子。结果寻常型银屑病患者编码HLA-C抗原第73位的丙氨酸序列、苏氨酸序列的频率与健康对照组差异有显著性，在有、无家族史的寻常型银屑病患者中差异亦有显著性。结论HLA-C第73位密码子与寻常型银屑病发病存在相关性；HLAC的第73位丙氨酸序列可能是该病的易感因素，而第73位苏氨酸序列似乎是该病的保护性因素。
목적탐토심상형은설병여인류백세포항원(HLA)복합체C기인좌위제73위안기산밀마자적상관성。방법채용취합매련반응—변성취병희선알응효전영—DNA은염、측서，검측료104례심상형은설병환자화110례건강대조자적HLA-C기인제73위안기산밀마자。결과심상형은설병환자편마HLA-C항원제73위적병안산서렬、소안산서렬적빈솔여건강대조조차이유현저성，재유、무가족사적심상형은설병환자중차이역유현저성。결론HLA-C제73위밀마자여심상형은설병발병존재상관성；HLAC적제73위병안산서렬가능시해병적역감인소，이제73위소안산서렬사호시해병적보호성인소。
Objective To explore the association of codons at position 73 of HLA-C with psoriasis vulgaris (PV) for the Hans in Hubei province. Methods Polymerase chain reaction (PCR)— denatured polyacrylamide gel electrophoresis— DNA silver stain technique and DNA sequencing of PCR products were employed to detect the codons at position 73 of HLA-C in 104 patients with psoriasis vulgaris and 110 healthy controls. Results ① The frequencies of nucleotide sequence coding alanine at position 73 of HLA-C antigens (73Ala) were significantly higher in patients (81.7％ ) than those in controls (53.6％ )[relative risk (RR)=3.87, χ2=19 12, P<0.005)]. However, the frequencies of nucleotide sequence coding threonine at position 73 of HLA－C antigens (73Thr) were significantly lower in patients (77.9％) than those in controls (89.1％ ), (RR=0.43, χ2=4. 92, P< 0.05). ② The frequencies of 73Ala was significantly higher in patients with a familial history (100％) of psoriasis than those in cases without familial history (77.6％ ), (χ2=5.39, P< 0.025). However, contrary results were obtained with the frequencies of 73Thr (57.9％ versus 82.4％ , χ2=5. 40, P< 0.025). Conclusions 73Ala of HLA－C gene may play a role in the susceptibility to PV, but 73Thr of HLA－C gene seems to be a protective factor to PV in the Hans.