CAJ | 학술논문

为探索甘菊[Chrysanthemum lavandulifolium（Fisch. ex Trautv.）Makino]在非生物胁迫下以及营养物质代谢中精准的生理、基因表达变化机理,研究通过筛选外植体的种类和营养液的浓度,建立了甘菊水培体系。结果表明：水培中,播种苗的成活率和生根率极显著高于1年生大苗和茎段插条;在长时间的水培中,使用标准Hoagland和1.5倍Hoagland培养液,其畸形叶率极显著低于1/2和1/4Hoagland培养液,并且株高极显著高于另外2个浓度。结合科研需求,认为组培播种苗、标准Hoagland营养液是甘菊水培的最佳外植体和培养条件。
위탐색감국[Chrysanthemum lavandulifolium（Fisch. ex Trautv.）Makino]재비생물협박하이급영양물질대사중정준적생리、기인표체변화궤리,연구통과사선외식체적충류화영양액적농도,건립료감국수배체계。결과표명：수배중,파충묘적성활솔화생근솔겁현저고우1년생대묘화경단삽조;재장시간적수배중,사용표준Hoagland화1.5배Hoagland배양액,기기형협솔겁현저저우1/2화1/4Hoagland배양액,병차주고겁현저고우령외2개농도。결합과연수구,인위조배파충묘、표준Hoagland영양액시감국수배적최가외식체화배양조건。
In order to explore the accurate mechanism of physiology and gene expression under abiotic stress and nutrient metabolism for Chrysanthemum lavadulifolium,we screened the explants and concentration of nutrient solution,and established the hydroponics culture system.The results indicate that in hydroponics culture,the survival rate and rooting rate are significantly higher in seedings rather than yearlings or cuttings;for a long time culture,the abnormal leaf rate is lower and the height is higher in normal and 1.5 times Hoagland solution than 1/2 or 1/4 Hoagland solution.Considering the actual demand in research,we choose seedings sown in MS medium,normal Hoagland solution as the best explants and culture medium.