CAJ | 학술논문

环介导等温扩增技术（LAMP）是由Notomi等建立的一种核酸扩增技术,该方法最大的优点是能够在63℃ ~65℃的等温条件下扩增特定DNA序列,并在30 ~60 min内可以观察到结果。LAMP已经成功用于许多病毒的检测,在本文中,主要概述与LAMP技术相关的最新发展现状以及国外应用该技术检测动物病毒方面的进展。
배개도등온확증기술（LAMP）시유Notomi등건립적일충핵산확증기술,해방법최대적우점시능구재63℃ ~65℃적등온조건하확증특정DNA서렬,병재30 ~60 min내가이관찰도결과。LAMP이경성공용우허다병독적검측,재본문중,주요개술여LAMP기술상관적최신발전현상이급국외응용해기술검측동물병독방면적진전。
Loop-mediated isothermal amplification（LAMP） is an amplification method developed by Notomi et al and has been applied successfully for the detection of many viruses.This paper introduced the current status of LAMP and recent developments,and the method applying in the diagnosis of animal viruses in abroad.