农业科学与技术:英文版
農業科學與技術:英文版
농업과학여기술:영문판
Agricultural Science & Technology
2011年
8期
1118-1120,1128
,共4页
王吉明%包文风%尚建立%马双武%樊辉
王吉明%包文風%尚建立%馬雙武%樊輝
왕길명%포문풍%상건립%마쌍무%번휘
甜瓜%种间杂交%SRAP分子标记
甜瓜%種間雜交%SRAP分子標記
첨과%충간잡교%SRAP분자표기
Cucumis ssp.%Interspecific hybridization%SRAP Markers
[目的]解释甜瓜属植物种间杂交特性。[方法]以150个薄皮甜瓜品种为母本,分别与甜瓜野生近缘种角瓜(Cucumismetuliferus)和西印度瓜(Cucumisanguria)进行种间杂交。[结果]以编号V2和V129的薄皮甜瓜与角瓜和西印度瓜杂交后能够坐果,但与角瓜杂交后种子败育,而与西印度瓜杂交后仅在果实底部有少量可育种子。采用SRAP分子标记技术对西印度瓜×V129杂交后代分析表明,1对引物(E14/M2)扩增出少量父本(西印度瓜)特征带,表明西印度瓜与V129已经在DNA分子水平上发生了交换。[结论]该研究为深入揭示该属植物中间杂交提供了资料。
[目的]解釋甜瓜屬植物種間雜交特性。[方法]以150箇薄皮甜瓜品種為母本,分彆與甜瓜野生近緣種角瓜(Cucumismetuliferus)和西印度瓜(Cucumisanguria)進行種間雜交。[結果]以編號V2和V129的薄皮甜瓜與角瓜和西印度瓜雜交後能夠坐果,但與角瓜雜交後種子敗育,而與西印度瓜雜交後僅在果實底部有少量可育種子。採用SRAP分子標記技術對西印度瓜×V129雜交後代分析錶明,1對引物(E14/M2)擴增齣少量父本(西印度瓜)特徵帶,錶明西印度瓜與V129已經在DNA分子水平上髮生瞭交換。[結論]該研究為深入揭示該屬植物中間雜交提供瞭資料。
[목적]해석첨과속식물충간잡교특성。[방법]이150개박피첨과품충위모본,분별여첨과야생근연충각과(Cucumismetuliferus)화서인도과(Cucumisanguria)진행충간잡교。[결과]이편호V2화V129적박피첨과여각과화서인도과잡교후능구좌과,단여각과잡교후충자패육,이여서인도과잡교후부재과실저부유소량가육충자。채용SRAP분자표기기술대서인도과×V129잡교후대분석표명,1대인물(E14/M2)확증출소량부본(서인도과)특정대,표명서인도과여V129이경재DNA분자수평상발생료교환。[결론]해연구위심입게시해속식물중간잡교제공료자료。
To understand the inter-hybridization between Cucumis ssp.plants,we used 150 melon varieties as female parents to cross with Cucumis metuliferus and Cucumis anguria.Only melon accessions V2 and V129 set fruits,but seeds from fruits V2(V129)×C.metuliferus were abortive.A few of seeds from the bottom of fruit V2(V129)×C.anguria were fertile.Sequence-related amplified polymorphism(SRAP)molecular markers were used to analyze the progenies of inter-specific hybridization between C.anguria and melon V129.One pair primer(E14/M2)was found effective in amplification on male parent characteristic bands from the hybrids,suggesting that some DNA exchange had happened between C.anguria and melon V129.This study provided data for analyzing the mechanism of inter-hybridization between Cucumis plants.