漳州师范学院学报：自然科学版
漳州師範學院學報：自然科學版
장주사범학원학보：자연과학판
Journal of ZhangZhou Teachers College（Natural Science)
2011年
2期
31-38
,共8页
多壁碳纳米管%过氧化苯甲酰%人绒毛膜促性腺激素%固体基质室温磷光免疫法%人体疾病的诊断
多壁碳納米管%過氧化苯甲酰%人絨毛膜促性腺激素%固體基質室溫燐光免疫法%人體疾病的診斷
다벽탄납미관%과양화분갑선%인융모막촉성선격소%고체기질실온린광면역법%인체질병적진단
multi-wall carbon nanotubes%benzoyl peroxide%human chorionic gonadotropin%solid substrate room temperature phosphorescence immunoassay%diagnosis of human diseases
基于过氧化苯甲酰（（C6H5CO）2O2）的强氧化性对多壁碳纳米管（multi-wall nanotubes,MWNTs）末端的切割效应,得到水溶性MWNTs＇,同时,利用聚丙烯酰胺（Polyacrylamide,PA）对MWNTs＇室温磷光（room temperature phosphorescence,RTP）的增敏效应,开发了水溶性PA-MWNTs＇磷光标记试剂.以MWNTs＇-PA标记人绒毛膜促性腺激素-β-C-末端肽单克隆抗体（human chorionic gonadotrophin-antibody,AbHCG）,获得可保持MWNTs＇的RTP优良特性AbHCG-MWNTs＇-PA标记产物.该AbHCG-MWNTs＇-PA不仅能与人绒毛膜促性腺激素（human chorionic gonadotrophin,HCG）发生定量的特异性免疫反应,而且免疫反应产物HCG-AbHCG-MWNTs＇-PA能发射更强的RTP信号,据此建立了固体基质室温磷光免疫法（solid substrate room temperature phosphorescence immunoassay,SSRTPIA）测定HCG的新方法.SSRTPIA的线性范围为0.040-20.0 pg HCG spot 1（样品体积0.40μL spot 1,对应浓度0.10-50.0 ng mL 1）,检出限为0.017 pg spot 1（447/613 nm,双抗夹心法）.该方法灵敏、准确、精密度好,已用于人血清中HCG含量的测定及人体疾病的诊断.
基于過氧化苯甲酰（（C6H5CO）2O2）的彊氧化性對多壁碳納米管（multi-wall nanotubes,MWNTs）末耑的切割效應,得到水溶性MWNTs＇,同時,利用聚丙烯酰胺（Polyacrylamide,PA）對MWNTs＇室溫燐光（room temperature phosphorescence,RTP）的增敏效應,開髮瞭水溶性PA-MWNTs＇燐光標記試劑.以MWNTs＇-PA標記人絨毛膜促性腺激素-β-C-末耑肽單剋隆抗體（human chorionic gonadotrophin-antibody,AbHCG）,穫得可保持MWNTs＇的RTP優良特性AbHCG-MWNTs＇-PA標記產物.該AbHCG-MWNTs＇-PA不僅能與人絨毛膜促性腺激素（human chorionic gonadotrophin,HCG）髮生定量的特異性免疫反應,而且免疫反應產物HCG-AbHCG-MWNTs＇-PA能髮射更彊的RTP信號,據此建立瞭固體基質室溫燐光免疫法（solid substrate room temperature phosphorescence immunoassay,SSRTPIA）測定HCG的新方法.SSRTPIA的線性範圍為0.040-20.0 pg HCG spot 1（樣品體積0.40μL spot 1,對應濃度0.10-50.0 ng mL 1）,檢齣限為0.017 pg spot 1（447/613 nm,雙抗夾心法）.該方法靈敏、準確、精密度好,已用于人血清中HCG含量的測定及人體疾病的診斷.
기우과양화분갑선（（C6H5CO）2O2）적강양화성대다벽탄납미관（multi-wall nanotubes,MWNTs）말단적절할효응,득도수용성MWNTs＇,동시,이용취병희선알（Polyacrylamide,PA）대MWNTs＇실온린광（room temperature phosphorescence,RTP）적증민효응,개발료수용성PA-MWNTs＇린광표기시제.이MWNTs＇-PA표기인융모막촉성선격소-β-C-말단태단극륭항체（human chorionic gonadotrophin-antibody,AbHCG）,획득가보지MWNTs＇적RTP우량특성AbHCG-MWNTs＇-PA표기산물.해AbHCG-MWNTs＇-PA불부능여인융모막촉성선격소（human chorionic gonadotrophin,HCG）발생정량적특이성면역반응,이차면역반응산물HCG-AbHCG-MWNTs＇-PA능발사경강적RTP신호,거차건립료고체기질실온린광면역법（solid substrate room temperature phosphorescence immunoassay,SSRTPIA）측정HCG적신방법.SSRTPIA적선성범위위0.040-20.0 pg HCG spot 1（양품체적0.40μL spot 1,대응농도0.10-50.0 ng mL 1）,검출한위0.017 pg spot 1（447/613 nm,쌍항협심법）.해방법령민、준학、정밀도호,이용우인혈청중HCG함량적측정급인체질병적진단.
Based on the cutting effect on the end of multi-wall carbon nanotubes（MWNTs） by taking advantages of the strong oxidation of benzoyl peroxide（（C6H5CO）2O2）,the water-soluble multi-wall nanotubes（MWNTs＇） were obtained.At the same time,we developed a new phosphorescent labeling reagent MWNTs＇-polyacrylamide（PA） utilizing the spiking effect of PA on the room temperature phosphorescence（RTP） of MWNTs＇.MWNTs＇-PA was used to label human chorionic gonadotropin-β-C-terminal peptide monoclonal antibody（AbHCG） to obtain labeling product AbHCG-MWNTs＇-PA which maintained good characteristics of RTP of the MWNTs＇.AbHCG-MWNTs＇-PA could take specific immunoreaction with human chorionic gonadotropin（HCG） to form HCG-AbHCG-MWNTs＇-PA,resulting in the increase of MWNTs＇ RTP signal.Thus,a new solid substrate room temperature phosphorescence immunoassay（SSRTPIA） for the determination of HCG was established.The linear range of SSRTPIA was 0.040-20.0 pg HCG spot-1（sample volume： 0.40 μL spot-1,corresponding concentration： 0.10-50.0 ng mL 1）,the detection limit（LD） was 0.017 pg spot 1 for sandwich way at 447/613 nm（λexmax/λemmax）.This sensitive,accurate and precise method was used for the determination of HCG and the diagnosis of human diseases.
