CAJ | 학술논문

背景:肝细胞移植作为治疗肝脏衰竭的一种有效方法在各种动物模型及临床应用中得到广泛证实,但供体短缺及移植肝细胞增殖困难等问题严重困扰着肝细胞移植的发展.研究表明骨髓间充质干细胞具有向肝细胞及胆管上皮细胞双向分化的潜能,而且具有强大的增殖能力,它作为一种新的细胞来源为解决上述难题提供了新思路.目的:以肝中叶注射移植骨髓间充质干细胞治疗新西兰在白兔肝脏衰竭,验证其效果.方法:健康雄性新西兰大白兔给予D氨基半乳糖24 h后,移植组于肝中叶注射骨髓间充质干细胞悬液3 mL(1x109L-1),对照组注射相同体积不含骨髓间充质干细胞的培养液.移植后48 h、72 h、1周、4周取外周血测定谷丙转氨酶、谷草转氨酶活性,移植后4周取肝脏做病理检测.结果与结论:移植后新西兰大白兔肝功能指标明显下降,存第4周时移植组谷丙转氨酶、谷草转氨酶活性明显低于对照组(P＜0.05).移植后4周对照组表现为肝素紊乱.肝细胞肿胀变性、灶性及大片坏死,伴出血及炎性细胞浸润.移植组显示肝小叶结构可辨认,点状坏死肝细胞间再生肝细胞较前增多,汇管区、中央静脉及坏死灶周围可见核/浆比例较大的小细胞,并向肝组织内延伸,可见灶性增生.
배경:간세포이식작위치료간장쇠갈적일충유효방법재각충동물모형급림상응용중득도엄범증실,단공체단결급이식간세포증식곤난등문제엄중곤우착간세포이식적발전.연구표명골수간충질간세포구유향간세포급담관상피세포쌍향분화적잠능,이차구유강대적증식능력,타작위일충신적세포래원위해결상술난제제공료신사로.목적:이간중협주사이식골수간충질간세포치료신서란재백토간장쇠갈,험증기효과.방법:건강웅성신서란대백토급여D안기반유당24 h후,이식조우간중협주사골수간충질간세포현액3 mL(1x109L-1),대조조주사상동체적불함골수간충질간세포적배양액.이식후48 h、72 h、1주、4주취외주혈측정곡병전안매、곡초전안매활성,이식후4주취간장주병리검측.결과여결론:이식후신서란대백토간공능지표명현하강,존제4주시이식조곡병전안매、곡초전안매활성명현저우대조조(P＜0.05).이식후4주대조조표현위간소문란.간세포종창변성、조성급대편배사,반출혈급염성세포침윤.이식조현시간소협결구가변인,점상배사간세포간재생간세포교전증다,회관구、중앙정맥급배사조주위가견핵/장비례교대적소세포,병향간조직내연신,가견조성증생.
BACKGROUND:Hepatocyte transplantation as an effective method for liver failure has been confirmed by animal models and clinical application.However,limited source and poor proliferation of hepatocyte graft limit its development.Studies have shown that bone marrow mesenchymal stem cells(MSCs)have potentials to differentiate into hepatocyte and bile epithelial celts,with strong proliferation.OBJECTIVE:To explore the therapeutic effect of bone marrow MSCs transplantation on liver failure of New Zealand white rabbits.METHODS:Adult male New Zealand rabbits were treated with D-galactosamine,and 3 mL hepatocyte suspension(1×109/L)was injected into the liver of transplantation group,but the control group was injected with the same volume of culture solution with no bone marrow MSCs.Alanine aminotransferase(ALT)and aspartate aminotransferase(AST)activity was detected 48,72 hours,1,4 weeks following transplantation,and pathological detection was performed at 4 weeks.RESULTS AND CONCLUSION:The liver functional index following transplantation of bone marrow-derived MSCs transplantation was significantly decreased,and ALT and AST activity at 4 weeks was significantly less than the control group(P ＜ 0.05).At 4,the transplantation group displayed disorderly hepatic cord,hepatocyte swollen and degeneration,necrosis,accompanied by bleeding and inflammatory cell infiltration.In addition,the hepatic lobule structure was detectable,and regenerative hepatocyte increased among necrotic hepatocyte;small cells with large ratio of nucleus and cytoplasm at header,central vein and surrouding necrosis focus extended to the liver tissues.