CAJ | 학술논문

以水热法合成的碲化镉(CdTe)纳米晶标记抗荧蒽抗体后,标记复合物的荧光强度增强,其分散性和稳定性良好.将此标记物用于直接竞争荧光免疫分析,测定了环境水样中荧蒽的含量.结果表明,在0.1～1000 μg/L范围内有良好的线性关系;抑制率IC_(50)为12.4 μg/L,检出限IC_(20)为13.1 ng/L.对水样进行加标回收实验,回收率在95.1%～111%之间;相对标准偏差小于9%.本方法准确可靠,结果满意,能够满足环境中微量环境激素类污染物的检测需要.
이수열법합성적제화력(CdTe)납미정표기항형은항체후,표기복합물적형광강도증강,기분산성화은정성량호.장차표기물용우직접경쟁형광면역분석,측정료배경수양중형은적함량.결과표명,재0.1～1000 μg/L범위내유량호적선성관계;억제솔IC_(50)위12.4 μg/L,검출한IC_(20)위13.1 ng/L.대수양진행가표회수실험,회수솔재95.1%～111%지간;상대표준편차소우9%.본방법준학가고,결과만의,능구만족배경중미량배경격소류오염물적검측수요.
The labeled compounds, CdTe was combined with anti-fluoranthene antibody, had good dispersion and stability with the fluorescence intensity enhancing. A direct competitive fluorescent immunoassay with CdTe-anti-fluoranthene antibody to detect fluoranthene in water sample in the environment was developed. The result showed that fluoranthene can be determined in the concentration range from 0.1 μg/L to 1000 μg/L with a correlation coefficient of 0.9983, a sensitivity of (IC_(50)) of 12.4 μg/L and a detection limit (IC_(20)) of 13.1 ng/L. Trace environmental pollutant in environmental water samples were successfully determined with a good accuracy and suitability. The recovery was between 95.1% and 111.0%, with relative standard deviation less than 9%.