CAJ | 학술논문

[目的]通过海7124和军棉1号构建的陆海杂交棉产量及重要农艺性状的QTL定位研究,筛选与产量及重要农艺性状紧密连锁的分子标记,提高棉花育种效率.[方法]选用陆地棉品种军棉1号和海岛棉品种海7124配制的一个包含148株F_2单株的群体,利用97对SSR标记构建了一个包含17个连锁群,长673.3 cM,标记间平均距离为17.3 cM的遗传图谱,覆盖整个棉花基因组12.2%.在此基础上对果枝始节、果枝台数、结铃数、单铃重、衣分和叶面积6个农艺性状进行了QTL定位研究.[结果]检测到3个稳定的QTL:1个与衣分相关的QTL,位于第4连锁群上;1个与果枝台数相关的QTL,位于第1连锁群上;1个与叶面积相关的QTL,位于第6连锁群上.[结论]这些QTL的效应值较大,对今后的棉花育种的分子辅助选择具有较大的意义.
[목적]통과해7124화군면1호구건적륙해잡교면산량급중요농예성상적QTL정위연구,사선여산량급중요농예성상긴밀련쇄적분자표기,제고면화육충효솔.[방법]선용륙지면품충군면1호화해도면품충해7124배제적일개포함148주F_2단주적군체,이용97대SSR표기구건료일개포함17개련쇄군,장673.3 cM,표기간평균거리위17.3 cM적유전도보,복개정개면화기인조12.2%.재차기출상대과지시절、과지태수、결령수、단령중、의분화협면적6개농예성상진행료QTL정위연구.[결과]검측도3개은정적QTL:1개여의분상관적QTL,위우제4련쇄군상;1개여과지태수상관적QTL,위우제1련쇄군상;1개여협면적상관적QTL,위우제6련쇄군상.[결론]저사QTL적효응치교대,대금후적면화육충적분자보조선택구유교대적의의.
[Objective and Method]In the present paper, a genetic linkage map was constructed using 148 F_2 individuals developed by cross of an upland cotton TM-1 and a sea-island cotton Hai7124. 97 SSR markers were chosen which showed stable polymorphism between two parents. This genetic linkage map covered a total of recombinational length of 673.3 cM with the average interval distance of 17.3 cM. Six agronomic traits including the first fruit node, node number, boll number, boll weight, lint percentage and leaf area were investigated. [Result]Three possible QTLs/loci with LOD≥2.5 were suggested. One QTL of lint percentage was identified on linkage group 4; one QTL of node number was located on linkage group 1; one QTL of leaf area was located on linkage group 6. [Conclusion]These QTLs had greater effect value which can be used effectively for MAS during the process of breeding in the future.