CAJ | 학술논문

精氨酸脱亚胺酶有较好的体内体外肿瘤生长抑制作用.通过对粪肠球菌(Enterococcus faecalis)NJ402菌株产精氨酸脱亚胺酶的发酵特性的研究,建立起代谢物的过程变化与精氨酸脱亚胺酶产生机理的内在联系.NJ402菌株生长过程中碳源物质代谢产生乳酸导致发酵体系pH的下降,而培养基中L-精氨酸的脱亚胺作用有利于发酵体系pH的稳定和菌体生长.进一步的研究表明,低pH生长环境有利于NJ402菌株产精氨酸脱亚胺酶,且精氨酸脱亚胺酶的产生与能量代谢无关.NJ402菌株产精氨酸脱亚胺酶受底物L-精氨酸的诱导,但该诱导作用受菌体生长体系pH的调控,即精氨酸脱亚胺酶的产生是低pH生长环境与L-精氨酸共同作用的结果.
정안산탈아알매유교호적체내체외종류생장억제작용.통과대분장구균(Enterococcus faecalis)NJ402균주산정안산탈아알매적발효특성적연구,건립기대사물적과정변화여정안산탈아알매산생궤리적내재련계.NJ402균주생장과정중탄원물질대사산생유산도치발효체계pH적하강,이배양기중L-정안산적탈아알작용유리우발효체계pH적은정화균체생장.진일보적연구표명,저pH생장배경유리우NJ402균주산정안산탈아알매,차정안산탈아알매적산생여능량대사무관.NJ402균주산정안산탈아알매수저물L-정안산적유도,단해유도작용수균체생장체계pH적조공,즉정안산탈아알매적산생시저pH생장배경여L-정안산공동작용적결과.
Arginine deiminase (ADI) is a potential anticancer agent.Studies on the characteristics of fermentation and mechanism of expression of ADI were the basic works for ADI availability.The process characteristics and the major intermediates in the fermentations of ADI in Enterococcus faecalis NJ402 were determined.A theory about the expression of ADI with the process of fermentation was proposed.In the earlier process of fermentation,the value of pH in fermentor was degressive to 5.5 for the secretion of lactic acid with catabolism of glucose in the cells of NJ402,while the value of pH was improved gradually with production of ammonia in the process of biocatalysis of ADI with arginine.The improved pH was available to the growth of NJ402.ADI expression in NJ402 was presumed to be contacted with the regulation of pH,and the degressive value of pH was the evocating agent.When NaOH was added to the system to keep the value of pH invariablenes,the specific activity of ADI was lower.ADI could not be evocative by the shortage of energy metabolism.Expression of ADI in NJ402 was induced by L-arginine and the process was regulated and controlled by pH in vitro.