CAJ | 학술논문

Objective:To study the effects of environmental estrogen-like chemical bisphenol A(BPA)on ovarian function with the model of the cultured human granulosa cells in vitro.Methods:The granulosa cells from healthy women were collected and cultured in DMEM with 10% heat-inacti-vated fetal bovine serum.BPA,final concentration 10-7 to 10-4 mol/L,was added to the cell-culture medium and treat cells for 48 hours.The levels of estrogen and progesterone in the supernatant of the cultured cells were meas-ured by DELFIA.Total RNA was extracted from the cultured cells.The expression levels of P450 scc and P450 arom mRNA were measured by RT-PCR.Results:The cultured human granulosa cells could express high levels of P450scc and P450arom.The levels of estrogen and progesterone increased after BPA treatment,expression of P450 arom mRNA was reduced significantly at 10-5 to 10-4mol/L of BPA,but the expression of P450 scc mRNA was increased at 10-6 to 10-5 mol/L of BPA.The levels of estrogen and progesterone in the supernatant of the cultured cells were not affected significantly by BPA at the same concentrations incubated for 48 hours.Conclusion:BPA could affect steroid hormone synthesis and transformation in granulosa cells,such as the ex-pression of P450 scc and P450 atom,so it can affect ovarian function.Although we did not find a significant effect of BPA on the final estrogen and progesterone levels in this studying model,noxious effects of BPA on ovarian function may be exist in the human granulose cells.