科技通报
科技通報
과기통보
BULLETIN OF SCIENCE AND TECHNOLOGY
2009年
2期
174-177
,共4页
朱承亮%叶雪飞%朱仕翔%何国庆
硃承亮%葉雪飛%硃仕翔%何國慶
주승량%협설비%주사상%하국경
嗜酸乳杆菌%高密度%发酵条件
嗜痠乳桿菌%高密度%髮酵條件
기산유간균%고밀도%발효조건
主要研究了嗜酸乳杆菌生长繁殖的环境条件(温度、接种量、起始pH等)和培养基组成对其影响.优化后确定了嗜酸乳杆菌的高密度培养条件为:起始pH值为5.8,培养温度为37℃,培养条件为亨-盖特厌氧培养,接种量为4%,培养时间为22 h.并且筛选了碳源,氮源及氮源的浓度.基配比为:2%乳清粉(w/v),1.5%牛肉膏(w/v),1.5%蛋白胨(w/v),0.058%硫酸镁(w/v),0.025%硫酸锰(w/v),0.22%乙酸钠(w/v),0.2%磷酸氢二钾(w/v).将该菌在此增菌培养基上培养,在37℃下培养22 h,菌数可达到1.17×1011cfu/mL.
主要研究瞭嗜痠乳桿菌生長繁殖的環境條件(溫度、接種量、起始pH等)和培養基組成對其影響.優化後確定瞭嗜痠乳桿菌的高密度培養條件為:起始pH值為5.8,培養溫度為37℃,培養條件為亨-蓋特厭氧培養,接種量為4%,培養時間為22 h.併且篩選瞭碳源,氮源及氮源的濃度.基配比為:2%乳清粉(w/v),1.5%牛肉膏(w/v),1.5%蛋白胨(w/v),0.058%硫痠鎂(w/v),0.025%硫痠錳(w/v),0.22%乙痠鈉(w/v),0.2%燐痠氫二鉀(w/v).將該菌在此增菌培養基上培養,在37℃下培養22 h,菌數可達到1.17×1011cfu/mL.
주요연구료기산유간균생장번식적배경조건(온도、접충량、기시pH등)화배양기조성대기영향.우화후학정료기산유간균적고밀도배양조건위:기시pH치위5.8,배양온도위37℃,배양조건위형-개특염양배양,접충량위4%,배양시간위22 h.병차사선료탄원,담원급담원적농도.기배비위:2%유청분(w/v),1.5%우육고(w/v),1.5%단백동(w/v),0.058%류산미(w/v),0.025%류산맹(w/v),0.22%을산납(w/v),0.2%린산경이갑(w/v).장해균재차증균배양기상배양,재37℃하배양22 h,균수가체도1.17×1011cfu/mL.
