CAJ | 학술논문

目的:探讨维生素A对角膜移植排斥反应后结膜杯状细胞的影响.方法:建立大鼠角膜移植排斥反应模型,随机分3组:A,B,C组为SD-wistar大鼠行同种异体角膜移植术,SD鼠为受体,wistar鼠为供体,其中A组为空白对照组,B组为(维生素A)诺沛凝胶滴眼液组,C组为1g/L地塞米松滴眼液组,另设D组为正常眼组.术后不同时间裂隙灯显微镜记录及比较各组角膜移植排斥指数(Rejection Index,RI).通过结膜组织学切片HE,PAS染色,并运用显微图像分析系统对结膜上皮杯状细胞数量及形态进行分析.结果:观察期间检测结膜杯状细胞发现,A,B,C组杯状细胞数量均少于D组(P＜0.01);A,B,C组内部比较,以C组数量为多,其次为B组,有显著性差异(P＜0.05).结论:维生素A具有保护角膜移植引起的结膜杯状细胞减少的作用.
목적:탐토유생소A대각막이식배척반응후결막배상세포적영향.방법:건립대서각막이식배척반응모형,수궤분3조:A,B,C조위SD-wistar대서행동충이체각막이식술,SD서위수체,wistar서위공체,기중A조위공백대조조,B조위(유생소A)낙패응효적안액조,C조위1g/L지새미송적안액조,령설D조위정상안조.술후불동시간렬극등현미경기록급비교각조각막이식배척지수(Rejection Index,RI).통과결막조직학절편HE,PAS염색,병운용현미도상분석계통대결막상피배상세포수량급형태진행분석.결과:관찰기간검측결막배상세포발현,A,B,C조배상세포수량균소우D조(P＜0.01);A,B,C조내부비교,이C조수량위다,기차위B조,유현저성차이(P＜0.05).결론:유생소A구유보호각막이식인기적결막배상세포감소적작용.
·AIM: To investigate the effect of vitamin A on the conjunctival goblet cells of rat after corneal transplantation.·METHODS: Rat graft rejection models of corneal transplantation were established. SD rats were receptor and Wistar rats were donors. After corneal allografts were performed, 48 SD rats were randomly divided into three groups, 16 rats in each group. Group A was blank control group; group B was treated by oculotect gel (containing vitamin A); group C was treated by 1g/L dexamethasone eyedrops. Besides, group D was normal unoperated eyes.Slit-lamp microscope was employed to record and compare rejection index (RI) of corneal transplantation. Through HE,PAS staining of conjunctival histological sections and image analysis system, the number and morphology of conjunctival goblet cells were observed and analyzed between operation group and normal group.·RESULTS: The HE, PAS staining detection showed that the number of conjunctival goblet cells in oculotect gel group,1g/L dexamethasone eyedrops group and control group is lower than that in normal group after surgery (P＜0.01). The number of conjunctival goblet cells in oculotect gel group and 1g/L dexamethasone eyedrops group is higher than that in control group (P＜0.05). The number of conjunctival goblet cells in 1g/L dexamethasone eyedrops group is higher than that in oculotect gel group (P＜0.05).·CONCLUSION: The results indicate that vitamin A may inhibit the decrease of conjunctival goblet cells after corneal allograft rejection in rats.