CAJ | 학술논문

背景:抵抗素是脂肪细胞分泌的一种多肽激素.而中心性肥胖时可引起胰岛素抵抗和导致2型糖尿病.目的:比较正常人腹部和大腿皮下脂肪组织抵抗素蛋白表达的情况,探讨抵抗素在中心性肥胖引起胰岛素抵抗中的作用.设计:对照观察实验.单位:华中科技大学同济医学院附属同济医院内分泌科.对象:选择2003-01/04华中科技大学同济医学院附属同济医院外科住院患者20例,根据留取脂肪组织的部位分腹部皮下脂肪组织组12例和大腿皮下脂肪组织组8例.方法:①所有患者测量血压、身高、体质量,计算体质量指数,体内脂肪百分比(按白种人数据推导的公式):男性=1.2×体质量(kg)+身高-2(m-2)+0.23×年龄-16.2;女性=1.2×体质量(kg)+身高-2(m-2)+0.23×年龄-5.4.②葡萄糖氧化酶法测定空腹血糖.③留取的脂肪组织标本用单去污裂解液提取脂肪组织中的蛋白质,考马斯亮蓝法测定蛋白质浓度,Western-blot测量脂肪组织抵抗素蛋白的表达水平.主要观察指标:两组患者的血压、体质量指数,体内脂肪百分比,空腹血糖,抵抗素蛋白的表达水平.结果:纳入患者20例,均进入结果分析.①两组的空腹血糖、收缩压、舒张压、体质量指数、体内脂肪百分比差异无显著性(P＞0.05).②腹部皮下脂肪组织抵抗素蛋白表达(A)为14 942±6 076,明显高于大腿皮下脂肪组织组39 421±6 087,二者比较差异显著(P＜0.01).结论:抵抗素蛋白在腹部皮下脂肪组织的表达高于腿部皮下脂肪组织,此结果对中心性肥胖并引起胰岛素抵抗和2型糖尿病的发生具有参考价值.
배경:저항소시지방세포분비적일충다태격소.이중심성비반시가인기이도소저항화도치2형당뇨병.목적:비교정상인복부화대퇴피하지방조직저항소단백표체적정황,탐토저항소재중심성비반인기이도소저항중적작용.설계:대조관찰실험.단위:화중과기대학동제의학원부속동제의원내분비과.대상:선택2003-01/04화중과기대학동제의학원부속동제의원외과주원환자20례,근거류취지방조직적부위분복부피하지방조직조12례화대퇴피하지방조직조8례.방법:①소유환자측량혈압、신고、체질량,계산체질량지수,체내지방백분비(안백충인수거추도적공식):남성=1.2×체질량(kg)+신고-2(m-2)+0.23×년령-16.2;녀성=1.2×체질량(kg)+신고-2(m-2)+0.23×년령-5.4.②포도당양화매법측정공복혈당.③류취적지방조직표본용단거오렬해액제취지방조직중적단백질,고마사량람법측정단백질농도,Western-blot측량지방조직저항소단백적표체수평.주요관찰지표:량조환자적혈압、체질량지수,체내지방백분비,공복혈당,저항소단백적표체수평.결과:납입환자20례,균진입결과분석.①량조적공복혈당、수축압、서장압、체질량지수、체내지방백분비차이무현저성(P＞0.05).②복부피하지방조직저항소단백표체(A)위14 942±6 076,명현고우대퇴피하지방조직조39 421±6 087,이자비교차이현저(P＜0.01).결론:저항소단백재복부피하지방조직적표체고우퇴부피하지방조직,차결과대중심성비반병인기이도소저항화2형당뇨병적발생구유삼고개치.
BACKGROUND: Resistin is an adipocyte-derived polypeptides. While central obesity can result in insulin resistance and type 2 diabetes mellitus.OBJECTIVE: To compare the expression of resistin protein in subcutaneous adipose tissue of normal human abdominal and thigh, and to investigate the role of resistin in central obesity bringing insulin resistance.DESIGN: Controlled observational experiment.SETTING: Department of Endocrinology, Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology.PARTICIPANTS: Totally 20 patients hospitalized between January and April 2003 at the Department of Surgery of Tongji Hospital Affiliated to Tongji Medical College, Huazhong University of Science and Technology were selected. All cases were divided into abdominal fat group with 12 cases and thigh fat group with 8 cases based on the site of fat tissue.METHODS: ①Blood pressure, height, body mass were measured and body mass index (BMI), percentage of body fat (BF%) in vivo (according to formulas induced by data from white people) were calculated: Male=1.2×body mass (kg)+height-2 (M-2)+0.23×age-16.2; Female =1.2× body mass (kg)×height-2 (m-2)+0.23×age-5.4. ②Fasting blood glucose was measured with glucose oxidase assay. ③Protein was isolated with lysis buffer and protein concentration was identified with Bradford method; The expression of resistin protein was measured with Western-blot method.MAIN OUTCOME MEASURES: Blood pressure, BMI, BF%; fasting blood glucose and expression of resistin protein of all cases of the two groups.RESULTS: Totally 20 patients were involved the result analysis. ①There were no significant difference in fast blood glucose, systolic blood pressure (SBP), diastolic blood pressure (DBP), body mass index (BMI) and BF%between two groups (P ＞ 0.05). ②Expression levels (A) of resistin protein in abdominal subcutaneous adipose tissues was (14 942±6 076), which was more higher than that in thigh subcutaneous adipose tissues (39 421±6 087). There was markedly significant difference (P ＜ 0.01).CONCLUSION: The expression of resistin protein is much greater in abdominal fat depots than in thigh fat depots, which has evaluative value for studying the episode of central obesity-induced insulin resistance and type 2 diabetes mellitus.