重组大肠杆菌分批-补料高密度发酵生产类人胶原蛋白的动力学
중조대장간균분비-보료고밀도발효생산류인효원단백적동역학
Kinetics of High Cell Density Fed-batch Culture of Recombinant Escherichia coli Producing Human-like Collagen
  • 万方数据
    中国化学工程学报（英文版） 중국화학공정학보（영문판）
    CHINESE JOURNAL OF CHEMICAL ENGINEERING
    2006年 2期 242-247 ,共6页

    花秀夫%范代娣%骆艳娥%张兮%施惠娟%米钰%马晓轩%尚龙安%赵桂仿

    화수부%범대제%락염아%장혜%시혜연%미옥%마효헌%상룡안%조계방



    human-like collagen%kinetics%high density fermentation%Escherichia coli


    The kinetics of batch and fed-batch cultures of recombinant Escherichia coli producing human-like collagen was investigated. In the batch culture, a kinetic model of a simple growth-association system was concluded without consideration of cell endogeneous metabolism. The cell lag time, the maximum specific growth rate and growth rates were set at (0.15, 0.2, 0.25h-1) by the method of pseudo-exponential feeding, and the expressions for the specific rate of substrate consumption, the growth kinetics and the product formation kinetics of each phase spectively. The model predictions are in good agreement with the experimental data.
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