CAJ | 학술논문

目的检测Ca(OH)2对体外培养的人牙髓细胞内Ca2+的影响。方法在RPMI1640培养液中加入Ca(OH)2、CaCl2和NaHCO4,培养第6代人牙髓细胞，用Fluo-3荧光探针负载后，再次用Ca(OH)2刺激，激光扫描共聚焦显微镜检测细胞内Ca2+。结果含Ca(OH)2培养液培养的牙髓细胞，再用Ca(OH)2刺激时胞内Ca2+升高；其他条件培养的细胞，Ca(OH)2刺激时Ca2+无变化。结论Ca(OH)2可导致人牙髓细胞内Ca2+升高。
목적검측Ca(OH)2대체외배양적인아수세포내Ca2+적영향。방법재RPMI1640배양액중가입Ca(OH)2、CaCl2화NaHCO4,배양제6대인아수세포，용Fluo-3형광탐침부재후，재차용Ca(OH)2자격，격광소묘공취초현미경검측세포내Ca2+。결과함Ca(OH)2배양액배양적아수세포，재용Ca(OH)2자격시포내Ca2+승고；기타조건배양적세포，Ca(OH)2자격시Ca2+무변화。결론Ca(OH)2가도치인아수세포내Ca2+승고。
Objective　To investigate the influence of calcium hydroxide to intracellular calcium ion of human pulp cells in vitro. Methods　The sixth generation of human pulp cells were maintained in RPMI 1640 medium supplemented with Fluo-3 probe and Ca(OH)2 for restimulation. The intracellular calcium ion concentration was detected by laser confocal microscope. Results　The intracellular calcium ion level in Ca(OH)2 pretreatment group increased after Ca(OH)2 stimulation,while it didn′t response to Ca(OH)2 stimulus in group without Ca(OH)2、CaCl2 or NaHCO4 pretreatment. Conclusion　It is important to pretreat the pulp cells with Ca(OH)2 in order to increase the intracellular calcium.