CAJ | 학술논문

WRKY蛋白参与植物对生物或非生物胁迫反应和一些发育、代谢过程,在植物中组成一个转录因子大家族.从水稻cDNA文库中分离到一个新的WRKY基因--OsWRKY52 cDNA,包括一个1 719 bp的开放读码框,推测编码一个由572个氨基酸组成的蛋白质,与燕麦(Avena sativa)AsWRKY1具有54%的氨基酸一致性.该基因被非亲和性稻瘟菌快速诱导.凝胶阻滞实验结果表明,原核表达的OsWRKY52能与水稻PR1a启动子上的W盒元件特异结合.采用酵母单杂交体系的方法证明了OsWRKY52具有转录激活活性,其丝氨酸岛、苏氨酸岛和C端的富酸性氨基酸区是负责转录激活的区域.这些结果提示OsWRKY52作为一个转录激活子,可能参与植物对稻瘟菌的应答反应.
WRKY단백삼여식물대생물혹비생물협박반응화일사발육、대사과정,재식물중조성일개전록인자대가족.종수도cDNA문고중분리도일개신적WRKY기인--OsWRKY52 cDNA,포괄일개1 719 bp적개방독마광,추측편마일개유572개안기산조성적단백질,여연맥(Avena sativa)AsWRKY1구유54%적안기산일치성.해기인피비친화성도온균쾌속유도.응효조체실험결과표명,원핵표체적OsWRKY52능여수도PR1a계동자상적W합원건특이결합.채용효모단잡교체계적방법증명료OsWRKY52구유전록격활활성,기사안산도、소안산도화C단적부산성안기산구시부책전록격활적구역.저사결과제시OsWRKY52작위일개전록격활자,가능삼여식물대도온균적응답반응.
WRKY proteins, a big family of transcription factors, are involved in regulation diverse developmental and physiological processes in plants. Here, a novel WRKY gene, OsWRKY52, was isolated from a rice cDNA library. This gene included an open reading frame of 1 719 bp in length, and the deduced polypeptide contained 572 amino acids,sharing 54% identity with a WRKY1 protein from Avena sativa. Expression of OsWRKY52 gene was induced rapidly by Magnaporthe grisea in the incompatible interaction with rice plant. OsWRKY52 protein, expressed prokaryotically bound specifically to W box cis elements derived from the promoter of a rice PR1a. Transcriptional activation assay was performed by a yeast one- hybrid method. Regions of transactivation were identified to be the N-terminal serine- and threonine-rich islands and the C-terminal acidic domain of OsWRKY52. These results suggest that OsWRKY52, as a transcription activator, may be involved in defense responses against Magnaporthe grisea in rice plants.