CAJ | 학술논문

目的研究肿瘤浸润性淋巴细胞（TIL）对肺癌细胞株（A549）的致凋亡作用及其作用机理，为临床应用TIL治疗肺癌提供依据.方法从肺癌病人胸水中用淋巴细胞分离液分离提取出TIL细胞，在含1000U/mlIL-2的完全培养基中培养20天后，将TIL细胞与靶细胞（肺癌细胞株）共同培养24小时，将培养细胞制成细胞涂片，经福尔马林固定，用原位末端脱氧核苷酸转移酶（TdT）介导的脱氧三磷酸嘧啶（duTP）末端标记法（TUNEL）原位的方法进行肺癌凋亡细胞的标记.结果肺癌肿瘤细胞凋亡指数（%）：经TIL作用组的凋亡指数为8.56±0.84，对照组凋亡指数为2.10±0.86，两组之间差别有非常显著性（p<0.01）.结论 TIL细胞诱导肺癌细胞的凋亡可能是TIL细胞对肺癌细胞杀伤作用的机理之一.
목적 연구종류침윤성림파세포（TIL）대폐암세포주（A549）적치조망작용급기작용궤리，위림상응용TIL치료폐암제공의거.방법 종폐암병인흉수중용림파세포분리액분리제취출TIL세포，재함1000U/mlIL-2적완전배양기중배양20천후，장TIL세포여파세포（폐암세포주）공동배양24소시，장배양세포제성세포도편，경복이마림고정，용원위말단탈양핵감산전이매（TdT）개도적탈양삼린산밀정（duTP）말단표기법（TUNEL）원위적방법진행폐암조망세포적표기.결과 폐암종류세포조망지수（%）：경TIL작용조적조망지수위8.56±0.84，대조조조망지수위2.10±0.86，량조지간차별유비상현저성（p<0.01）.결론 TIL세포유도폐암세포적조망가능시TIL세포대폐암세포살상작용적궤리지일.
Objective The function of induced apoptosis of tumorinfiltrating lymphocytes (TIL) to lung carcinoma cell line (A549) was studied in this paper.The basic data of study was provided for the clinical application of TIL's treatment to lung carcinoma.Methods The TIL cells were separated from pleural effusion of lung carcinoma patients by Ficoll-Hypaque (1.077±0.001) After cultured for 20 days in completed medium with 1000U/ml IL-2, TIL cells co-cultured with target cells (carcinoma cell line) for 24 hours. Then cultured cells were smeared on the slides, and fixed the slides in 10% formalin.Apoptotic carcinoma cells were labeled by TdT-mediate dUTP nick end labeling(TUNEL) method.Results Apoptotic index (%)of carcinoma cells was:8.56±0.84 in TIL experimental group, 2.10±0.86 in control group.There was different significance between the above two groups(p<0.01).Conclusions Apoptosis of lung carcinoma cells induced by TIL cells may be one of the mechanisms of TIL cells killed lung carcinoma cells.