CAJ | 학술논문

目的：研究芦荟多糖(AP)的抗瘤作用及其机制。方法：采用移植性S180肿瘤小鼠(实体瘤)和H22肝癌小鼠(腹水瘤)，单独ip或ivAP，或与环磷酰胺(CTX)、阿霉素(ADM)、氟脲嘧啶(FU)联合用药。连续给药10天后，S180肿瘤小鼠剥取瘤块，称重，减毒试验则于处死前采血，化验血象，计算脾指数和胸腺指数；H22肝癌小鼠停药后观察生存时间，或于停药后24小时取血，放射免疫分析法测IL-2和TNF含量。结果：AP25mg/kg·d、50mg/kg·d两个剂量单独给药时，能显著降低S180肉瘤移植小鼠瘤块重量，显著延长H22肝癌移植小鼠的生存时间；与三种抗肿瘤药物合用时，均有不同程度的增效作用，且可减轻化疗药物的副作用；可显著提高荷瘤鼠血清中IL-2、TNF含量。结论：AP对移植性S180肿瘤小鼠和H22肝癌小鼠具有抗肿瘤作用及化疗增效和减毒的双重作用，其机理可能是诱生IL-2、TNF及提高机体免疫力。
목적：연구호회다당(AP)적항류작용급기궤제。방법：채용이식성S180종류소서(실체류)화H22간암소서(복수류)，단독ip혹ivAP，혹여배린선알(CTX)、아매소(ADM)、불뇨밀정(FU)연합용약。련속급약10천후，S180종류소서박취류괴，칭중，감독시험칙우처사전채혈，화험혈상，계산비지수화흉선지수；H22간암소서정약후관찰생존시간，혹우정약후24소시취혈，방사면역분석법측IL-2화TNF함량。결과：AP25mg/kg·d、50mg/kg·d량개제량단독급약시，능현저강저S180육류이식소서류괴중량，현저연장H22간암이식소서적생존시간；여삼충항종류약물합용시，균유불동정도적증효작용，차가감경화료약물적부작용；가현저제고하류서혈청중IL-2、TNF함량。결론：AP대이식성S180종류소서화H22간암소서구유항종류작용급화료증효화감독적쌍중작용，기궤리가능시유생IL-2、TNF급제고궤체면역력。
Objectrive:To study the antitumor activity and mechanism of aloepolysaccharides (AP).Methods:AP was administered ip or iv to Sarcoma 180(S180) bearing mice or Hepatoma22(H22) bearing mice solely or combining with CTX,FU and ADM respectively.10 days later,for S180 mice,the blood was analyzed,the tumor was peeled off and weighed,and the spleen index,thymus index was calculated.For H22 bearing mice,the survival rate was observed or the IL-2,TNF content in serum was tested.Results:25 mg/kg·d or 50 mg/kg· d AP group could evidently reduce the tumor weight of S180 bearing mice and prolong the survival time of H22 bearing mice.AP also could improve the antitumor effects of CTX,ADM,FU,and lessen the chemotherapy side-effects.Furthermore,AP could improve the level of IL-2,TNF in the serum of mice bearing Si80 or H22.Conclusion:AP has the effects of antitumor,enhancing the artitumor activity of chemotherapy drugs and lessening their side-effects.This effect was possibly derived from inducing IL-2 and TNF producing in body and improving the immunity activity.