CAJ | 학술논문

目的　探讨电离辐射对小鼠腹腔巨噬细胞阴离子通道活动的影响及康复新对其的作用。方法　采用电生理膜片钳离子单通道记录方法。结果　用调理的zymosan刺激腹腔巨噬细胞后，阴离子通道开放的阳性率在受照射组显著减少，且受刺激至通道开放的时间间隔显著延长。受照射组较正常对照组通道开放概率显著降低、开放时间常数缩短而关闭时间常数延长，康复新对正常对照组离子通道活动的影响不显著，但可使照射组的通道开放有一定程度的加强。结论　电离辐射抑制巨噬细胞膜上阴离子通道的开放可能是其抑制巨噬细胞功能的一个重要途径。康复新可减弱电离辐射的这一抑制作用。
목적　탐토전리복사대소서복강거서세포음리자통도활동적영향급강복신대기적작용。방법　채용전생리막편겸리자단통도기록방법。결과　용조리적zymosan자격복강거서세포후，음리자통도개방적양성솔재수조사조현저감소，차수자격지통도개방적시간간격현저연장。수조사조교정상대조조통도개방개솔현저강저、개방시간상수축단이관폐시간상수연장，강복신대정상대조조리자통도활동적영향불현저，단가사조사조적통도개방유일정정도적가강。결론　전리복사억제거서세포막상음리자통도적개방가능시기억제거서세포공능적일개중요도경。강복신가감약전리복사적저일억제작용。
Objective　To study the effects of irradiation and W11-a12，a kind of repair-promoting drug，on anion-selective channel in membranes of mouse peritoneal macrophage. Methods　The activity of anion-selective channel was recorded from cell-attached patches with patch clamp techniques． Results　The effects of irradiation on anion-selective channel in membranes of peritoneal macrophage included：①decreasing the mean number of activated channels by the presence of zymosan; ②prolonging the mean time from stimulus to the opening of channels; ③depressing the opening of channels by decreasing open-state probability，shortening open-time and prolonging close-time． The effects of irradiation could partly be depressed by W11-a12. Conclusion　Irradiation will depress the anion-selective channel of peritoneal macrophage, which may be an important way to depress the function of macrophage．