CAJ | 학술논문

以绿色荧光蛋白GFP基因为报道基因，用花粉管通道和农杆菌介导的转化方法将外源基因导入棉花(Gossypium hirsutum L.)，分别获得转化幼胚、幼苗和转化愈伤组织。用手持紫外灯结合显微镜检术能够快速地对转化子进行活体筛选鉴定，比用GUS检测方法有明显的优越性。本研究不但为花粉管通道转化法的可行性提供了新的证据，同时也建立了GFP用于棉花基因工程研究的检测技术体系。
이록색형광단백GFP기인위보도기인，용화분관통도화농간균개도적전화방법장외원기인도입면화(Gossypium hirsutum L.)，분별획득전화유배、유묘화전화유상조직。용수지자외등결합현미경검술능구쾌속지대전화자진행활체사선감정，비용GUS검측방법유명현적우월성。본연구불단위화분관통도전화법적가행성제공료신적증거，동시야건립료GFP용우면화기인공정연구적검측기술체계。
With the Green Fluorescent Protein gene (GFP) as a reporter gene, the transgenic embryos, seedlings and calli of cotton(Gossypium hirsutum L.) were obtained by the method of pollen tube pathway and Agrobacterium-mediated techniques separately. The GFP gene under the control of the 35s Cauliflower Mosaic Virus promoter produced bright–green fluorescence easily detectable and screenable in cotton tissue by fluorescence microscopy and a hand-held ultraviolet lamp. The screenable marker aided and facilated the rapid segregation of individual transformation events, drastically reduced the quantity of tissue to be handled. The GFP can be screened in vivo without destroying the materials, so it is more practical and useful than GUS. The use of GFP could advance the development of cotton gene engineering.