遗传学报
遺傳學報
유전학보
ACTA GENETICA SINICA
2001年
2期
166-170
,共5页
仇艳光%沈银柱%黄占景%赵保存%何聪芬%秘彩莉
仇豔光%瀋銀柱%黃佔景%趙保存%何聰芬%祕綵莉
구염광%침은주%황점경%조보존%하총분%비채리
普通小麦%T型细胞质雄性不育%线粒体%分子杂交
普通小麥%T型細胞質雄性不育%線粒體%分子雜交
보통소맥%T형세포질웅성불육%선립체%분자잡교
以小麦T型细胞质雄性不育系75-369A和相应保持系75-369B为材料，用限制性内切酶BamH、EcoR、Hind完全酶解，以Oenothera mtDNA atp6，小麦线粒体基因nad3／rps12、cox1为探针进行Southern杂交。杂交结果表明，75-369A和75-369B在这3个基因上或附近有显著的组织结构差异，推测这些差异可能影响了线粒体基因组的正常功能，最终引起了75-369A雄性不育。
以小麥T型細胞質雄性不育繫75-369A和相應保持繫75-369B為材料，用限製性內切酶BamH、EcoR、Hind完全酶解，以Oenothera mtDNA atp6，小麥線粒體基因nad3／rps12、cox1為探針進行Southern雜交。雜交結果錶明，75-369A和75-369B在這3箇基因上或附近有顯著的組織結構差異，推測這些差異可能影響瞭線粒體基因組的正常功能，最終引起瞭75-369A雄性不育。
이소맥T형세포질웅성불육계75-369A화상응보지계75-369B위재료，용한제성내절매BamH、EcoR、Hind완전매해，이Oenothera mtDNA atp6，소맥선립체기인nad3／rps12、cox1위탐침진행Southern잡교。잡교결과표명，75-369A화75-369B재저3개기인상혹부근유현저적조직결구차이，추측저사차이가능영향료선립체기인조적정상공능，최종인기료75-369A웅성불육。
mtDNAs of T type wheat cytoplasmic male sterile lines 75-369A and their maintainers 75-369B were isolated and digested completely with restriction endonuclease BamH, EcoR, Hind. The mitochondrial genomic difference between CMS line and maintainers were revealed by Southern hybridization with probes of atp6 from Oenothera and nad3／rps12, cox from wheat mitochondria. Southern hybridization showed the differences between these two lines. Discussion was made on the change of the DNA composition and the male sterile cytoplasm.
