CAJ | 학술논문

目的研究地昔帕明(DMI)对大鼠脑胶质瘤C6的增殖抑制和凋亡诱导作用，为三环类抗抑郁药作为肿瘤辅助治疗提供新的理论和实验依据。方法用MTT比色法测定C6细胞活力，用透射电镜、流式细胞术(FCM)检测细胞凋亡，用免疫组化法分析bcl-2蛋白的表达。结果 DMI对C6细胞的增殖具有明显的浓度依赖性抑制作用，IC50(95%置信区间)为20.7(17.3～24.2)μmol*L－1，细胞阻滞于G0～G1期。DMI(40 μmol*L-1)使细胞发生典型的凋亡形态改变，D NA含量分析显示G0峰左侧出现亚二倍体细胞凋亡峰，呈浓度依赖性，蛋白合成抑制剂CHX 可显著抑制DMI诱导的细胞凋亡。同时，DMI(10 μmol*L－1)可下调细胞bcl-2蛋白的表达。结论 DMI体外对C6细胞的增殖具浓度依赖性抑制作用，并诱导细胞凋亡。
목적연구지석파명(DMI)대대서뇌효질류C6적증식억제화조망유도작용，위삼배류항억욱약작위종류보조치료제공신적이론화실험의거。방법용MTT비색법측정C6세포활력，용투사전경、류식세포술(FCM)검측세포조망，용면역조화법분석bcl-2단백적표체。결과 DMI대C6세포적증식구유명현적농도의뢰성억제작용，IC50(95%치신구간)위20.7(17.3～24.2)μmol*L－1，세포조체우G0～G1기。DMI(40 μmol*L-1)사세포발생전형적조망형태개변，D NA함량분석현시G0봉좌측출현아이배체세포조망봉，정농도의뢰성，단백합성억제제CHX 가현저억제DMI유도적세포조망。동시，DMI(10 μmol*L－1)가하조세포bcl-2단백적표체。결론 DMI체외대C6세포적증식구농도의뢰성억제작용，병유도세포조망。
AIM　To study the effect of desipramin e (DMI) on proliferation inhibition and apoptosis induction of rat glioma C6 cel ls. METHODS　Cell proliferation w as measured by MTT colorimetric assay and cells undergoing apoptosis were determ ined by electron microscope and flow cytometry. The expression of bcl-2 was eva luated by immunohistochemistry. RESULTS　DMI could result in the c oncentration-dependent inhibition of C6 cell proliferation and lead to arrest i n G0～G1 phase of cell cycle. The value of IC50 and 95% confidence lim its were 20.7(17.3～24.2) μmol*L－1.DMI（40 μmol*L－1）-indu ced apoptosis showed classical apoptotic morphology and the hypodiploid peak ap peared on the histogram of FCM in a concentration-dependent manner, which could be abrogated by cycloheximide(1.8 μmol*L－1). Meanwhile, DMI (10 μmol *L－1) could down-regulate the expression of apoptosis associated gene b cl-2. CONCLUSION　DMI could inhibit cell proliferation in a con centration dependent manner and induce typical apoptosis of C6 cells.