CAJ | 학술논문

目的探讨粘附分子P-选择素和细胞间粘附分子-1(ICAM-1)在肾缺d再灌注损伤中的作用，以及P-选择素单克隆抗体的阻断意义。方法建立肾缺d再灌注损伤大鼠模型，观察P-选择素单抗处理前后的大鼠肾组织中P-选择素及ICAM-1表达，及其丙二醛(MDA)含量、超氧化物歧化酶(SOD)活性和细胞凋亡等变化。结果缺d再灌注后，大鼠肾组织中P-选择素和ICAM-1先后明显表达，且MDA含量增加和SOD活性下降，出现明显的细胞凋亡及组织学病理改变。经给予P-选择素单抗处理，肾组织中P-选择素和ICAM-1表达受到抑制，MDA含量降低和SOD活性增高，细胞凋亡减少及组织学病理改变减轻。结论粘附分子P-选择素和ICAM-1参与了肾缺d再灌注损伤机制，以单抗抑制P-选择素可明显减少肾内炎症细胞浸润，脂质过氧化反应，细胞凋亡及组织损伤。
목적탐토점부분자P-선택소화세포간점부분자-1(ICAM-1)재신결d재관주손상중적작용，이급P-선택소단극륭항체적조단의의。방법건립신결d재관주손상대서모형，관찰P-선택소단항처리전후적대서신조직중P-선택소급ICAM-1표체，급기병이철(MDA)함량、초양화물기화매(SOD)활성화세포조망등변화。결과결d재관주후，대서신조직중P-선택소화ICAM-1선후명현표체，차MDA함량증가화SOD활성하강，출현명현적세포조망급조직학병리개변。경급여P-선택소단항처리，신조직중P-선택소화ICAM-1표체수도억제，MDA함량강저화SOD활성증고，세포조망감소급조직학병리개변감경。결론점부분자P-선택소화ICAM-1삼여료신결d재관주손상궤제，이단항억제P-선택소가명현감소신내염증세포침윤，지질과양화반응，세포조망급조직손상。
Objective To investigate the role of P-selectin and intercellular adhesive molecule-1 (ICAM-1) inrenal ischemia-reperfusion injury, as well as the preventive effect of anti-P-selectin monoclonal antibody (mAb).Method P-selectin and ICAM-1 expression in renal tissue, cell apoptosis, MDA contents and SOD activities were detected in rat model of renal ischemia-reperfusion injury. Some ischemia-reperfusion rats were treated with anti-P-selectin mAb. Resalts Histopathologic damage, up-regulated expression of P-selectin and ICAM-1, cell apoptosis, increased MDA contents and declined SOD activities were found in renal tissues following renal ischemia-reperfusion injury, while these changes became less conspicuous in animal treated with anti-P-selectin mAb. Couclusian P-selectinand ICAM-1 might mediate inflammatory cell infiltration, cell apoptosis and lipid peroxidation and contribute to renalischemia-reperfusion injury, which could be inhibited by the anti-P-selectin mAb. (Shanghai Med J, 2001,24:85-88)