分析化学
分析化學
분석화학
CHINESE JOURNAL OF ANALYTICAL CHEMISTRY
2001年
2期
192-194
,共3页
5-氟尿嘧啶%脱氧核糖核酸%血清%白蛋白
5-氟尿嘧啶%脫氧覈糖覈痠%血清%白蛋白
5-불뇨밀정%탈양핵당핵산%혈청%백단백
紫外光谱、荧光光谱、圆二色谱法等方法研究了抗癌药物5-氟尿嘧啶(5-fluorouracil,5-FU)与DNA、血清蛋白的相互作用及某些二价金属离子对其作用的影响。求得了5-FU与HSA、BSA的结合常数。
紫外光譜、熒光光譜、圓二色譜法等方法研究瞭抗癌藥物5-氟尿嘧啶(5-fluorouracil,5-FU)與DNA、血清蛋白的相互作用及某些二價金屬離子對其作用的影響。求得瞭5-FU與HSA、BSA的結閤常數。
자외광보、형광광보、원이색보법등방법연구료항암약물5-불뇨밀정(5-fluorouracil,5-FU)여DNA、혈청단백적상호작용급모사이개금속리자대기작용적영향。구득료5-FU여HSA、BSA적결합상수。
The interaction of 5-fluorouracil (5-FU) with deoxyribonucleic acid (DNA) and serum albumin and the influence of some metal ions on the interaction have been studied by using spectral methods of UV-absorption,fluorescence and circular dichroism. It was shown that the interaction of 5-FU with DNA and serum albumin was effected by some metal ions. The binding costant of 5-FU with serum albumin was found to be KHSA = 4.38 × 103(mol/L)-1 and KBSA =4.95 × 103(mol/L)-1 and the distance between the bound 5-FU and the tryptophan residue was also determined to be rHSA = 3.53 nm, rBSA = 3.51 nm.
