CAJ | 학술논문

目的了解膀胱移行细胞癌中抑癌基因p16缺失及其蛋白表达情况。方法应用双重PCR和免疫组化S-P法对31例膀胱移行细胞癌组织分别进行缺失和突变检测。结果发现有7例存在p16基因的缺失，缺失率为22.6%。其中有1例外显子1缺失，有5例外显子2缺失，1、2外显子共缺失的有1例。p16蛋白阴性率为38.7%。结论抑癌基因p16的变异及其蛋白的不表达与膀胱移行细胞癌的发生有一定关系。
목적 료해방광이행세포암중억암기인p16결실급기단백표체정황。방법 응용쌍중PCR화면역조화S-P법대31례방광이행세포암조직분별진행결실화돌변검측。결과 발현유7례존재p16기인적결실，결실솔위22.6%。기중유1예외현자1결실，유5예외현자2결실，1、2외현자공결실적유1례。p16단백음성솔위38.7%。결론 억암기인p16적변이급기단백적불표체여방광이행세포암적발생유일정관계。
Objective To investigate the deletion of antioncogene p16 andexpression of its protein in bladder transitional cell carcinoma.Methods 31 cases of bladder transitional cell carcinoma were detected by duplex PCR and immunohistochemical staining.Results The gene deletions were found in seven samples.The deletion retio of p16 gene was 22.6%.Five cases had deletion in exon 2.One case had deletion in exon 1 and one had deletion in both exon 1 and exon 2 .The expression negative rates of p16 were 38.7%.Conclusion.The results suggest that the deletions of antioncogene p16 and the no expression of p16 were associated withbladder transitional cell carcinoma.