CAJ | 학술논문

为研究部分显性的机制，用12个黑腹果蝇醇脱氢酶（DADH）基因内无效突变（Adhn）体作为材料。这些已知序列的突变体［包括单碱基置换或基因内小段缺失（9～16个碱基）］，用来分析肽的合成，二聚体的形成和杂二聚体酶活性［1］。杂合体中酶的部分表达和很广范围显性表达（从几乎完全隐性到高度显性）具多重机制。在10%乙醇的高度胁迫下，所有12个Adhn型果蝇中都观察到醇耐受性的部分显性表达。无效突变表达的遗传背景是杂合果蝇醇耐受性的主要决定因素。X射线诱导的突变体较ENU诱导的突变体有较高的平均显性，形成无功能杂二聚体的突变体具有最高显性。
위연구부분현성적궤제，용12개흑복과승순탈경매（DADH）기인내무효돌변（Adhn）체작위재료。저사이지서렬적돌변체［포괄단감기치환혹기인내소단결실（9～16개감기）］，용래분석태적합성，이취체적형성화잡이취체매활성［1］。잡합체중매적부분표체화흔엄범위현성표체（종궤호완전은성도고도현성）구다중궤제。재10%을순적고도협박하，소유12개Adhn형과승중도관찰도순내수성적부분현성표체。무효돌변표체적유전배경시잡합과승순내수성적주요결정인소。X사선유도적돌변체교ENU유도적돌변체유교고적평균현성，형성무공능잡이취체적돌변체구유최고현성。
To study the mechanisms of partial dominance, 12 intragenic alcohol dehydrogenase null mutations (Adhn) in Drosophila melanogaster were used as a model systerm. These known sequenced mutations, either single base substitutions or small intragenic deletions (9～16bp) were analyzed for peptide production, dimer formation and enzymatic activity of the heterodimer. Multiple mechanisms leading to partial enzyme expression in heterozygotes and a wide range of dominance varying from almost complete recessive to a high degree of dominance were found. An expression of partial dominance for alcohol tolerance was observed for all 12 Adhn under the high stress of 10% ethanol. The genetic background on which the null mutations are expressed is a major determinant for alcohol tolerance of the heterozygous adult flies. The mutations induced by X-ray had higher average dominance than that of ENU杋nduced mutations, and the mutations that formed nonfunctional heterodimers had the highest dominance.